摘要
目的探讨丙型肝炎病毒(HCV)对高尔基体蛋白73(GP73)表达的影响。方法选取2015年3月至2016年12月期间上海市浦东新区公利医院收治的68例HCV患者为研究对象,以同期74例健康体检者为对照组,采用酶联免疫吸附试验(ELISA)检测两组受检者血清中GP73的含量,逆转录-聚合酶链式反应(RT-PCR)和免疫印迹法检测Huh7.5.1细胞中GP73 m RNA和蛋白的表达情况,ELISA检测细胞上清中GP73的含量,用SPSS19.0软件进行统计学分析。结果 HCV组患者的GP73血清含量为(146.5±12.8)ng/m L,明显高于健康对照组的(45.2±2.7)ng/m L,差异有统计学意义(P<0.05);感染HCV的Huh7.5.1细胞中GP73 m RNA和蛋白相对表达量分别为(1.204±0.143)和(1.174±0.136),明显高于对照细胞的(0.306±0.019)和(0.298±0.015),差异均具有统计学意义(P<0.05);HCV感染的细胞上清中GP73含量为(85.6±10.3)ng/m L,明显高于对照细胞的(39.5±8.3)ng/m L,差异具有统计学意义(P<0.05)。结论 HCV可能通过上调GP73的表达来参与HCC的形成,但HCV调节GP73表达的具体分子机制尚待进一步研究。
Objective To explore the effect of hepatitis C virus (HCV) on the expression of Golgi protein 73(GP73). Methods A total of 68 HCV patients who were diagnosed in Gongli Hospital of Pudong New Area of shanghaifrom March 2015 to December 2016 were selected as research objects, and 74 healthy persons were enrolled as controlgroup in the same period. Serum level of GP73 was measured by enzyme-linked immunosorbent assay (ELISA) betweenthe two groups. Reverse transcription PCR (RT-PCR) and Western blot were used to measure mRNA and protein expres-sion of GP73 in Huh7.5.1 cells. The GP73 level in the supernatant of Huh7.5.1 was measured by ELISA, and the resultswere statistically analyzed by SPSS19.0 software. Results The serum level of GP73 was (146.5±12.8) ng/mL in HCVgroup, which was significantly higher than (45.2±2.7) ng/mL in the healthy control group (P〈0.05); The relative expres-sion of GP73 mRNA and protein in Huh7.5.1 cells infected by HCV were respectively (1.204±0.143) and (1.174±0.136),which were significantly higher than (0.306±0.019) and (0.298±0.015) in the control cells (P〈0.05); The GP73 level inthe supernatant of Huh7.5.1 cells infected by HCV was (85.6±10.3) ng/mL, which was significantly higher than (39.5±8.3) ng/mL in the control cells (P〈0.05). Conclusion HCV can promote the synthesis and secretion of GP73 in vitroand in vivo. It may participate in the formation of hepatocellular carcinoma by upregulating the expression of GP73, butthe molecular mechanism of HCV regulating GP73 expression needs further study.
出处
《海南医学》
CAS
2018年第5期657-659,共3页
Hainan Medical Journal
基金
上海市卫生和计划生育委员会科研课题项目(编号:201440459)
上海市医学重点专科建设计划项目(编号:ZK2015B16)
浦东新区卫生系统重点学科群建设资助项目(编号:PWZxq2017-15)
关键词
丙型肝炎病毒
高尔基体蛋白73
表达
Hepatitis C virus (HCV)
Golgi protein 73 (GP73)
Expression