一测多评法同时测定小儿麻甘颗粒中5种成分含量

Simultaneous Determination of 5 Constituents in Xiao'er Magan Granule by HPLC-QAMS

目的:建立小儿麻甘颗粒中盐酸麻黄碱、苦杏仁苷、甘草苷、黄芩苷、甘草酸铵5种成分的含量一测多评法。方法:以苦杏仁苷为内参照物,建立盐酸麻黄碱、甘草苷、黄芩苷、甘草酸铵与苦杏仁苷的相对校正因子,分别采用一测多评法与外标法测定5种成分的含量,比较两种方法测得结果的差异,验证一测多评法的可行性。结果:苦杏仁苷与盐酸麻黄碱、甘草苷、黄芩苷、甘草酸铵的相对校正因子分别为1.237,1.318,1.327,0.884,一测多评法与外标法测得结果无显著差异,相对误差小于0.3%。结论:一测多评法运用于小儿麻甘颗粒的含量测定准确可行。

Objective： To develop a method of quantitative analysis of multi-components by single marker（ QAMS） for the determination of five constituents（ ephedrine hydrochloride,amygdalin,liquiritin,baicalin and ammonium glycyrrhizinate） in Xiao＇er Magan granule. Methods： Amygdalin was used as the internal reference substance,and the relative correlation factors（ RCF） of ephedrine hydrochloride,liquiritin,baicalin and ammonium glycyrrhizinate to amygdalin were calculated and evaluated. The contents of the five constituents were determined by the external standard method（ ESM） and QAMS,respectively. The content results determined by the two methods were compared and the feasibility of QAMS method was verified. Results： The RCF between amygdalin and the other contents was 1. 237,1. 318,1. 327 and 0. 884,respectively. There were no significant differences in the results between QAMS and ESM with the relative errors less than 0. 3%. Conclusion： The QAMS method is accurate and feasible for the simultaneous determination of Xiao＇er Magan granule.