GC法同时测定肚痛丸中6种有效成分的含量

Simultaneous Determination of Six Effective Components in Dutong Pills by GC

目的:建立同时测定肚痛丸中6种成分含量的气相色谱方法。方法:色谱柱为HP-5柱(30 m×0.32 mm,0.25μm);采取程序升温,载气为氮气,流速为2.0 ml·min^(-1),进样量为1μl,分流比为5∶1,进样口温度为280℃,检测器(FID)温度为300℃。结果:桂皮醛、乙酸龙脑酯、木香烃内酯、去氢木香内酯、厚朴酚、和厚朴酚分别在32.28~516.40μg·ml^(-1)(r=0.999 3)、27.06~433.00μg·ml^(-1)(r=0.999 2)、25.65~410.40μg·ml^(-1)(r=0.999 3)、26.10~417.60μg·ml^(-1)(r=0.999 3)、24.01~384.20μg·ml^(-1)(r=0.999 0)、18.32~293.10μg·ml^(-1)(r=0.999 4)范围内呈良好的线性关系;平均加样回收率分别为99.71%(RSD=0.67%)、99.34%(RSD=1.18%)、100.16%(RSD=0.34%)、100.40%(RSD=0.39%)、99.32%(RSD=1.22%)、99.58%(RSD=0.58%)(n=6)。结论:该方法操作简便,灵敏度高,准确度好,可为控制该制剂的质量提供依据。

Objective： To establish a GC method for the imultaneous determination of cinnamaldehyde,bornyl acetate,costunolide,dehydrocostus lactone,magnolol and honokiol in Dutong pills. Methods： The determination was performed on an HP-5 column（ 30 m × 0. 32 mm,0. 25 μm） with programmed temperature. The carrier gas was nitrogen with the flow rate of 2. 0 ml·min^（-1）. The injection volumn was 1 μl and the sample split ratio was 5∶ 1. The inlet temperature was 280 ℃. The detector was a flame ionization detector with temperature at 300 ℃. Results： The linear ranges were 32. 28-516. 40 μg · ml^-1 for cinnamaldehyde（ r = 0. 999 3）,27. 06-433. 00 μg·ml^-1 for bornyl acetate（ r = 0. 999 2）,25. 65-410. 40 μg·ml^-1 for costunolide（ r = 0. 999 3）,26. 10-417. 60 μg·ml^-1 for dehydrocostus lactone（ r = 0. 999 3）,24. 01-384. 20 μg·ml^-1 for magnolol（ r = 0. 999 0） and 18. 32-293. 10 μg·ml^-1 for honokiol（ r = 0. 999 4）. The average recovery was 99. 71%（ RSD = 0. 67%）,99. 34%（ RSD = 1. 18%）,100. 16%（ RSD = 0.34%）,100. 40%（ RSD = 0. 39%）,99. 32%（ RSD = 1. 22%） and 99. 58%（ RSD = 0. 58%）（ n = 6）,respectively. Conclusion： The method is simple,sensitive and accurate,can be used to supplement the insufficient quality control of Dutong pills.