cagE在胃肠疾病患者中的分布及其临床意义

Distribution of cagE gene in Helicobacter pylori infected gastrointestinal patients and its clinical significance

目的 :研究 cag E在幽门螺杆菌 (H elicobacter pylori,Hp)感染的不同胃肠疾病患者中的分布及其与 Hp感染相关疾病的关系。 方法 :合成 cag EU 1- cag EU 2和 cag E3- cag E4两组引物 ,应用聚合酶链反应 (PCR)法扩增 14 5株临床分离培养的Hp菌株 cag E片段。 结果 :Hp临床菌株的 cag EU 1- cag EU 2 PCR产物总检出率为 75 .9%(110 / 14 5 ) ,慢性胃炎、十二指肠溃疡、胃溃疡、复合溃疡中检出率分别为 6 9.4 %、85 .4 %、76 .5 %、75 .0 %,溃疡组略高于胃炎组 ,但差异无显著性 (P>0 .0 5 )。cag E3- cag E4总检出率为 4 2 .1%(6 1/ 14 5 ) ,慢性胃炎、十二指肠溃疡、胃溃疡、复合溃疡中检出率分别为 38.9%,4 7.9%,35 .3%,5 0 %,差异无显著性 (P>0 .0 5 ) ;cag E总检出率为 79.3%(115 / 14 5 )。 结论 :cag E在不同的消化道疾病患者感染的Hp中均有较高的检出率 ,在不同疾病中的分布无特异性 ,cag

Objective:To investigate the distribution of Helicobacter pylori (Hp) cagE gene in Chinese with gastroduodenal diseases and its relationship with Hp infected diseases. Methods:A 335 bp fragment(primer set cagEU1 cagEU2) and 1 176 bp fragment (primer set cagE3 cagE4) of cagE were amplified by polymerase chain reaction (PCR) in 145 Hp strains isolated from patients with chronic gastritis(72), duodenal ulcer(48), gastric ulcer(17), gastric and duodenal ulcer(8). Results:CagEU1 cagEU2 was present in 75.9% Hp strains isolated from patients, and 69.4% of pateints with chronic gastritis, 85.4%, 76.5%, 75.0% with duodenal ulcer, gastric ulcer, gastric and duodenal ulcer, respectively. The prevalence of cagE3 cagE4 was 42.1%, and it was 38.9%,47.9%, 35.3% and 50% in patients with chronic gastritis, duodenal ulcer, gastric ulcer, gastric and duodenal ulcer, respectively. Totally, cagE was found in 79.3% of Hp strains. Conclusion:Most of the Hp strains in Chinese patients are cagE positive regardless of the clinical outcome, and cagE can't be a single marker for discrimination of Hp strains with respect to a specific gastroduodenal disease.