巴曲酶对急性缺血性脑卒中患者外周血中黏附分子表达的影响

Effect of bartroxobin on adhesion molecule expression in peripheral blood of patients with acute ischemic stroke

目的 :观察巴曲酶对急性缺血性脑卒中患者外周血白细胞黏附分子和血清中可溶性黏附分子表达水平的影响。 方法 :治疗组 18例 ,予巴曲酶 3d(2 0 BU )并予其他常规治疗 ;对照组 18例 ,除未予巴曲酶外 ,余用药同巴曲酶治疗组。采用流式细胞术和酶联免疫吸附法测定患者卒中后不同时间外周血中性粒细胞和单核细胞 CD11b、CD18、CD6 2 L、CD5 4的表达以及血清中可溶性 ICAM- 1、VCAM- 1的水平。结果 :与对照组比 ,巴曲酶治疗组卒中患者在发病 2 4 h后中性粒细胞 CD11b的表达下降 (P<0 .0 5 ) ,单核细胞 CD11b、中性粒细胞和单核细胞 CD18、CD6 2 L、CD5 4的表达及血清中可溶性 ICAM- 1、VCAM- 1的水平均无显著性变化。 结论 :巴曲酶对急性缺血性脑卒中患者外周血黏附分子的表达的影响有限 。

Objective:To observe the expression changes of adhesion molecules in peripheral blood leukocytes and serum soluble adhesion molecules in acute ischemic stroke after treatment with bartroxobin. Methods:Treatment group( n =8) was given bartroxobin (20 BU in 3 d) and other routine treatment;Control group( n =18) was similar to treatment group except for bartroxobin.The expression of CD11b,CD18,CD62L,CD54 on polymorphonuclear and monocyte were measured by flow cytometry, soluble ICAM 1 and VCAM 1 were measured by enzyme linked immunosorbent assay in consecutive patients[within 12,24,48 h( P <0.05) after stroke onset]. Results:Compared with control group, the mean fluorescence intensity of CD11b on polymorphonuclear decreased significantly in bartroxobin treatment group 48 h after stroke onset.No significant changes of CD11b expression on monocyte,CD18,CD62L,CD54 expression on polymorphonuclear and monocyte, serum level of soluble ICAM 1,VCAM 1 were detected. Conclusion:Decreasing adhesion molecule expression may not involve in the chief mechanism of bartroxobin in treatment of acute stroke.