富血小板血浆对中波紫外线照射成纤维细胞活性氧的影响

Platelet-rich plasma ameliorates photo-aging phenotype of fibroblasts by adjusting the generation of reac- tive oxygen species

目的探讨富血小板血浆（platelet—richplasma，PRP）对于光老化成纤维细胞活性氧产生的调节作用．以及对光老化成纤维细胞形态与衰老的影响。方法将成纤维细胞分为uVB组和uVB＋PRP组。采用适宜浓度的PRP处理中波紫外线（UVB）反复照射构建的体外细胞光老化模型，造模结束24h后，倒置显微镜观察2组细胞形态以及衰老染色阳性率；紫外线单次照射后，荧光显微镜检测2组活性氧的表达量；流式细胞仪检测实验组与对照组活性氧产生的相对荧光强度的大小。结果PRP能够明显改善由于UVB照射所引起的成纤维细胞肥大铺展的外观，能够明显减少活性氧的产生，降低活性氧的相对荧光强度。结论PRP能够通过降低uVB照射引起的光老化成纤维细胞的氧化应激水平，减少活性氧的产生量，改善光老化成纤维细胞的相关衰老表型。

Objective To explore the effect of platelet rich plasma （PRP） on the generation of reactive oxygen species （ROS） and the phenotypes of photo-aging fibroblasts. Methods A photoaging cell model by repeating UVB irradiation was treated using appropriate concentration of PRP; Cell morphology and the rate of aging dying were observed under inverted microscope 24 hours later after establishment of the cell model; The expression of ROS between experimental and control group was detected using fluorescence microscope after single UVB irradiation. The relative intensity of fluores- cence was analyzed using flow cytometry. Results PRP could ameliorate the large and sprawl appear- ance of photoaging fibroblasts obviously, reduce the generation of ROS as well as decrease the relative intensity of ROS. Conclusions PRP can decrease the level of intracellular oxidative stress caused by UVB irradiation, reduce the generation of ROS and ameliorate the senescence-like phenotypes of pho- toaging fibroblasts.