乳复宁颗粒调节乳腺增生症家兔激素水平以及VEGF蛋白表达变化的研究

Effects of Rufuning granules on hormone levels and the expression of VEGF protein in mammary gland hyperplasia rabbits

目的:研究乳复宁颗粒改善乳腺增生疾病症状的作用及机制。方法:将48只家兔按体质量随机分为6组,每组8只,即正常对照组、模型对照组、乳癖消片组、乳复宁颗粒高、中、低剂量组。给予雌性未孕家兔注射苯甲酸雌二醇,建立乳腺增生病理模型,测定家兔乳房直径和乳头高度,并检测各组家兔血清中泌乳素(PRL)、雌二醇(E2)、孕酮(P)的含量,镜下观察乳腺组织病理形态学变化。同时测定家兔乳腺组织中血管内皮生长因子(VEGF)蛋白的表达改变。结果:乳复宁颗粒高、中剂量组可降低乳腺增生症家兔乳房直径和乳头高度(P<0.01),降低血清中PRL、E_2的含量(P<0.01),增加血清中P的含量;减少乳腺小叶数、小叶腺泡数,减轻管腔扩张和乳腺间质纤维结缔组织增生;同时降低乳腺组织中VEGF的过度表达。结论:乳复宁颗粒可能通过调整雌孕激素比例及降低VEGF的表达来改善乳腺增生的症状。

AIM： To investigate the mechanism and the effects of Rufuning granules on improving the symptom of mammary gland hyperplasia（ MGH）.METHODS： Forty-eight rabbits were randomly divided into six groups（ n = 8）, normal control group,model group,Rupixiao tablets group,Rufuning-high dose,medium dose and low dose groups.The model of MGH was induced by injecting estradiol benzoate in unpregnant female rabbits. Rabbits in the normal control received saline injection only.The breast diameter and nipple height of rabbits were determined. The contents of prolactin（ PRL）,estradiol（ E_2） and progesterone（ P） in serum in each group were detected. Pathologic changes in morphology due to MGH in rabbits were observed by microscopy. Meanwhile,the expression of VEGF protein in rabbit breast tissue was determined. RESULTS：The breast diameter and nipple height of rabbits were reduced after treatment in high and medium doses of Rufuning granules group（ P 0. 05）. The contents of PRL and E_2 in serum were reduced（ P 0. 05） while those of progesterone in serum were improved（ P 0. 05）. The performance of histology breast hyperplasia was significantly improved. The number of mammary gland flocculus and lobular gland bubble and lumen expansion were reduced in rabbit mammary. Mammary gland hyperplasia of interstitial fibrous connective tissue was also significantly alleviated as compared with those in model group. Meanwhile,VEGF over-expression in breast tissue was reduced. CONCLUSION： Rufuning granules may improve symptom of MGH by adjusting the ratio of estrogen and progesterone and reducing VEGF over-expression.