樟芝分级沉淀的多糖组分对链脲佐菌素构建的糖尿病小鼠的降糖作用及体外抑制α-糖苷酶活性的研究

Inhibition of alpha glucosidase activity in vitro and the hypoglycemic effect of antrodia camphorata polysaccharide for diabetic mice construction by streptozotocin

目的:探索乙醇分级沉淀得到的三种多糖组分对于链脲佐菌素(STZ)构建的糖尿病小鼠的降血糖作用及体外α-糖苷酶的活性抑制作用。方法:利用乙醇对总多糖进行分级沉淀,得到三种多糖。将小鼠进行STZ腹腔注射造膜,构建糖尿病小鼠,将小鼠分为对照组、模型组、药物组(分为三组,为三种多糖),通过药物干预14 d后,检测小鼠空腹血糖(FBG)、胰岛素(FINS)、胰高血糖素(GC)的表达,计算胰岛素抵抗指数(HOMA-IR),取小鼠胰岛组织检测超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)的表达。体外进行α-糖苷酶的活性抑制的检测。结果:三种多糖均有不同程度的降糖作用,其作用与胰岛素、胰高血糖素的表达有关,多糖对于胰岛组织中SOD、MDA、GSH-Px的表达也有不同程度的干预作用。体外研究表明,多糖对于α-糖苷酶有着显著的抑制作用,且有剂量依赖性。结论:樟芝多糖对于糖尿病小鼠有着显著的治疗作用,而不同组分的多糖效果不一,作用机制多样。樟芝多糖是一种具有潜力的多糖类药物,值得深入研究和开发。

AIM： To investigate the hypoglycemic effect and inhibition of alpha glucosidase activity in vitro of three polysaccharide obtained from ethanol fractionation precipitation on diabetic mice constructed with streptozotocin（ STZ）. METHODS： Three polysaccharides were obtained by fractionation and precipitation of total polysaccharides with ethanol.The diabetic mice were constructed by intraperitoneal injection of STZ and divided into control group,model group, drug group（ divided into three groups,three kinds of polysaccharides）. Fourteen days after drug intervention,the fasting blood glucose（ FBG）,insulin（ FINS）,glucagon（ GC）,expression of insulin resistance index（ HOMA-IR）,SOD,MDA and GSH-Px in pancreatic islets of mice were detected. RESULTS： Three kinds of polysaccharides had hypoglycemic effects at different degrees,and their effects were related to the expression of insulin and glucagon. Polysaccharides also interfered with the expression of SOD,MDA and GSH-Px in islet tissues at different degrees. In vitro studies showed that polysaccharides significantly inhibited alpha glucosidase and the effects were dosedependent. CONCLUSION： The polysaccharide has significant therapeutic effect on diabetic mice,but the effects of different components of polysaccharides are different,and the mechanism is diverse.Antrodia camphorate polysaccharide is a promising polysaccharide drug,which deserves further study and development.