汉黄芩素对1型糖尿病小鼠的干预作用及对肝脏p62dok表达水平影响的研究

Intervention impact of Wogonin on type 1 diabetic mice and its influence on p62dok expression in liver

目的探究汉黄芩素(Wogonin)对T1DM小鼠的干预作用,以及对小鼠肝脏中p62dok表达水平的影响。方法 SPF级别雄性C57BL/6小鼠50只,随机分为5组。对照组(Con,n=10);STZ组(n=40),STZ造模成功后,选取7只为造模组。再根据予不同剂量的Wogonin分为5、10、20mg/kg共3个干预组,每组10只。分别测定各组FPG水平,通过腹腔葡萄糖耐量试验(IPGTT)检测其糖耐量情况;ELISA检测各组血清胰岛素水平;Western blot检测各组肝组织中接头蛋白1(Dok1)、Dok2以及蛋白激酶B(AKT)蛋白的表达水平。结果 Wogonin可降低STZ诱导的T1DM小鼠FPG水平[(12.55±1.31)vs(7.24±0.49)vs(6.22±0.69)mmol/L,P<0.05],改善其糖耐量情况,且可使STZ诱导的T1DM小鼠血清胰岛素水平恢复正常;Wogonin可刺激STZ诱导的T1DM小鼠肝脏内Dok1[(0.29±0.09)vs(0.68±0.14)vs(0.79±0.13)]和Dok2[(0.32±0.08)vs(0.61±0.07)vs(0.84±0.12)]的表达水平升高(P<0.05);10mg/kg Wogonin组和20mg/kg Wogonin组FIns含量高于STZ造模后组(P<0.05)。结论 Wogonin可缓解STZ诱导的T1DM小鼠的高血糖症和低胰岛素血症,且能刺激糖尿病小鼠肝脏组织中Dok1和Dok2的表达提高。

Objective To explore the Intervention impact of Wogonin on type 1 diabetic mice and its influence on p62 dok expression in liver. Methods Fifty SPF level male C57 BL/6 mice were randomly divided into 5 groups：control group（Con,n=10）,diabetes group（STZ group,n=40）.Diabetic model was successfully made by STZ.After that,7 were chosen as model group,and then STZ group was subdivided into three subgroups according to different doses of Wogonin：5,10,and 20 mg/kg subgroup（n=10/subgroup）.Fasting blood glucose levels were measured in all the subjects.And intraperitoneal injection of glucose tolerance test（IPGTT）was used to evaluate he glucose tolerance.Serum insulin level was tested by ELISA kits.Dok1,Dok2 and AKT protein expression level in liver tissue were measured by western blot. Results Wogonin could significantly reduce FPG in STZ induced type 1 diabetic mice[（12.55±1.31）vs（7.24±0.49）vs（6.22±0.69）mmol/L,P〈0.05].Wogonin could improve the glucose tolerance,and restore the serum insulin levels to normal in type 1 diabetic mice induced by STZ.Wogonin could increase Dok1[（0.29±0.09）vs（0.68±0.14）vs（0.79±0.13）]and Dok2[（0.32±0.08）vs（0.61±0.07）vs（0.84±0.12）]expression levels in the liver of type 1 diabetic mice induced by STZ（P〈0.05）.Serum insulin level was significantluy higher in10 mg/kg Wogonin intervention group and20 mg/kg Wogonin intervention group than in model group（P〈0.05）,indicating that serum insulin level increased after Wogonin intervention. Conclusion Wogonin could significantly alleviate hyperglycemia and hyperinsulinemia in STZ induced type 1 diabetic mice,and could stimulate the expression of Dok1 and Dok2 in the liver tissue of diabetic mice.