非生物逆境胁迫下甘蔗CBL1和CBL6基因表达分析

Expression Analysis of CBL1 and CBL6 Genes in Sugarcane under Abiotic Stress

CBLs蛋白是植物一类特殊的Ca2+信号感受器,在植物遭受逆境胁迫过程中发挥重要的调节作用。文章利用NCBI数据库信息获得2个甘蔗CBL家族基因,分别为SsCBL1和SsCBL6。SsCBL1基因包含一个完整的642 bp的完整开放阅读框,编码213个氨基酸;SsCBL6基因包含一个完整的672 bp完整开放阅读框,编码223个氨基酸。通过生物信息学分析方法对SsCBL1和SsCBL6基因编码蛋白结构进行分析。结果显示两者均无跨膜结构域并且都包含4个EF-hand结构域。进化树分析显示SsCBL1和SsCBL6与其他植物的CBL1和CBL6亲缘关系较近,具有较高的保守性。实时荧光定量PCR检测结果发现,这两个基因在低氮、低磷、低钾、干旱、盐和ABA胁迫过程中其表达均发生变化。SsCBL1基因在干旱和盐胁迫过程中,表达量上调幅度较大,而SsCBL6基因在低钾、干旱和盐处理时表达量上调幅度大。实时定量分析结果表明SsCBL1和SsCBL6基因在多种胁迫下发挥重要的调控作用。

CBLs（calcineurin B-like Proteins） is a type of calcium sensors in plant,which plays an important role in response to stress.We derived 2 sugarcane CBL family genes from NCBI database,namely SsCBL1 and SsCBL6.SsCBL1 gene contained a complete open reading frame of 642 bp,encoding a protein with 213 amino acids.SsCBL6 gene contained a complete open reading frame of 672 bp,encoding a protein with 223 amino acids.The structure information of SsCBL1 and SsCBL6 were analyzed by bioinformatics.The results showed that both of them did not contain transmembrane domain but contained four EF-hand functional domains.The phylogenetic tree analysis indicated that SsCBL1 and SsCBL6 were closely related to CBL1 and CBL6 in other plants,and they were highly conservative.The results of real-time quantitative PCR demonstrated that the two genes changed in low nitrogen,low phosphorus,low potassium,drought,salt and ABA stress.The expression of SsCBL1 increased significantly under drought and salt stress,while the expression of SsCBL6 increased under low potassium,drought and salt treatment.Real time quantitative analysis suggested that SsCBL1 and SsCBL6 might play important regulatory roles under a variety of stresses.