巴西橡胶树NAC基因家族5个成员的荧光原位杂交物理定位

Physical Location of FISH of Five Members in NAC Gene Family from Hevea brasiliensis

NAC转录因子是在植物的境胁迫应答、生长发育以及激素调节中具有重要的功能一类转录调控因子。为了研究巴西橡胶树NAC基因家族中5个成员(HbNAC1,HbNAC2,HbNAC3,HbNAC24,HbNAC33)在染色体的具体位置,以巴西橡胶树(Hevea brasiliensis)无性系热研7-33-97品种为材料,利用荧光原位杂交技术对其进行了物理定位分析。结果表明:HbNAC1、HbNAC3、HbNAC33基因分别位于第5号、第9号和第2号染色体的短臂上,对应的信号位点距着丝粒平均百分距分别为73.09、27.42和26.53;HbNAC24、HbNAC2基因分别定位在巴西橡胶树的第6号和第3号染色体的长臂区域,其所对应着的着丝粒与信号位点之间的百分距离平均值为63.67和66.10。本研究通过物理定位确定这些基因在染色体上的具体位置和相互关系,在基因组学、分子辅助育种等研究方面有很好的应用前景。

The NAC transcription factors are important transcription regulators in environmental stress response,growth and development,and hormone regulation in plants.In order to reveal specific location of the five members of NAC gene family（HbNAC1,HbNAC2,HbNAC3,HbNAC24,HbNAC33） on chromosomes of Hevea brasiliensis,Hevea brasiliensis cultivar Reyan 7-33-97 was selected as materials,the physical localization analysis of which was carried out by fluorescence in situ hybridization（FISH）.The results showed that HbNAC1,HbNAC3,and HbNAC33 genes were located on the short arm of chromosome 5,chromosome 9 and chromosome 2,respectively,and the average distance between the signal and the true silk were 73.09,27.42 and 26.53,respectively;HbNAC2 and HbNAC24 genes were located on the long arm of chromosome 3 and chromosome 6,and the average distance between the signal and the true silk were 66.10 and 63.67.The location and interrelationship of these genes on chromosomes were determined by physical position in this study,which might have a good application prospects in genomics,molecular assisted breeding and other research aspects.