辣椒6421子叶外植体离体培养

In Vitro Culture of Cotyledon Explants of Capsicum annuum L. 6421

以辣椒自交系6421子叶外植体为实验材料,通过筛选6-BA浸泡预处理浓度、优化不定芽诱导培养基、芽伸长培养基和生根培养基配方来提高辣椒子叶离体培养效率。结果表明:6-BA浸泡预处理子叶外植体最佳浓度为50 mg/L,最佳不定芽诱导培养基为MS+4.0 mg/L 6-BA,外植体浸泡处理1 min后接种至该培养基中,培养15 d后不定芽诱导率达93.21%;最佳不定芽伸长培养基为MS+6.0 mg/L 6-BA+0.1 mg/L IAA,培养20 d后芽伸长率可达23.21%;最佳生根培养基为1/2 MS+0.25 mg/L IAA+0.25 mg/L IBA,培养30 d后生根率可达90.12%。

The cotyledon explants of pepper inbred line 6421 were selected as the experimental material. The culture efficiency of pepper cotyledon in vitro was optimized by screening 6-BA immersion pretreatment concentration, optimizing adventitious bud induction medium, bud elongation medium and rooting medium formula. The results showed that the optimum concentration of 6-BA soaking pretreatment for cotyledon explants was 50 mg/L, and the optimum medium for adventitious bud induction was MS with 4.0 mg/L 6-BA. Inoculate the explants which had been soaked for 1 min into the medium, and the induction rate of adventitious buds reached 93.21% after 15 d culture. MS medium with 6.0 mg/L 6-BA and 0.1 mg/L IAA was the optimum medium for the elongation of adventitious bud. The rates of shoot elongation from shoot buds were 23.21% within 20 days of culture initiation. 1/2 MS medium with 0.25 mg/L IAA and 0.25 mg/L IBA was the best rooted medium and the rates of root induction was 90.12% after 30 days of culture initiation.