不同胁迫博落回根中生物碱含量变化及相关基因表达量分析

Changes of Alkaloids Content and Expression of Related Genes for Macleaya by Treating for Different Stress Conditions

本研究通过对PEG6000模拟干旱以及机械受损等方法胁迫博落回,采用HPLC方法检测博其根中原阿片碱、别隐品碱、血根碱、白屈菜红碱的含量,结果 30%PEG6000模拟干旱以及机械受损均可使博落回根中终产物生物碱血根碱、白屈菜红碱含量上升,中间产物原阿片碱、别隐品碱含量下降;且血根碱、白屈菜红碱合成基因DBOX、P6H表达量上调;用实时荧光定量RT-PCR检测生物碱合成相关基因的表达量,血根碱、白屈菜红碱合成基因DBOX、P6H表达量上调。适当干旱以及机械受损有利于博落回根中血根碱、白屈菜红碱积累,为其在干旱、石漠化地区种植以及在种植过程中结合家畜喂养提供理论依据。

In this study, Macleaya cordata were stressed by PEG6000 simulating drought and mechanical damaging, and HPLC was used to detect the contents ofprotopine, allocryptopine, sanguinarine and chelerythrine. It was found that the contents of end products in Macleaya root such as sanguinarine and chelerythrine increased by 30% PEG6000 sximulating drought stressing and mechanical damaging. On the contrary, the contents of intermediate products such as protopine and allocryptopine reduced. Meanwhile, there was up-regulation in expression of synthetic genes DBOX and P6H in sanguinarine and chelerythrine. After RT-PCR was used to detect related gene expression, the expression of synthetic genes DBOX and P6H in sanguinarine and chelerythrine were up-regulated. The appropriate drought stress and mechanical damage might be advantageous to sanguinarine and chelerythrine accumulation in root of Macleaya, which provided the theoretical basis for Macleaya planting in arid and desertification areas and combining livestock feeding and Macleaya planting.