摘要
目的探讨FTO基因rs17817449(T/G)和SEC16B基因rs574367(C/A)单核苷酸多态性(SNP)与代谢综合征(MS)的相关性。方法纳入体检人群1224例,根据美国国家胆固醇教育计划成人治疗方案第三次报告的修订标准(ATPⅢ*),分为代谢综合征(MS)组(共520例)和非MS组(共704例)。SNP检测采用扩增阻碍突变系统(ARMS)结合Taq Man-MGB探针的实时荧光定量PCR方法。结果 FTO-rs17817449的TT、TG和GG等位基因分布频率在MS组和非MS组间差异不具有统计学意义(P>0.05)。与非MS组比较,MS组SEC16B-rs574367的CA型(35.6%vs31.0%)、AA型(5.0%vs 2.7%)分布频率显著升高(P<0.05)。控制年龄、性别的Logistic回归分析显示,SEC16B-rs574367是MS患病的独立危险因素(OR值为1.341,95%CI:1.089~1.651,P=0.006),其中CA型携带者较CC型发生MS的危险性为1.249倍(95%CI:0.971~1.606),AA型携带者的危险性为2.271倍(95%CI:1.215~4.247)。结论 SEC16B-rs574367与MS具有显著相关性,是MS患病的遗传易感性标志位点。
Objective To explore the association of single nucleotide polymorphisms （SNP）in PTO- rs17817449and SEC16B-rs574367 with metabolic syndrome （MS）. Methods 1224 health routing body examinesubjects were enrolled, and were divided into MS group （ 520 cases） and no- MS group （ 704 cases） according to the modified National Cholesterol Education Program Adult Treatment Panel m criteria （ATP Ⅲ ＊ ）. The genotyping of the cohortwere then performed by amplification refractory mutation system （ARMS） and combined a TaqMan-MGB fluorogenic probeas signal report in a real time PCR. Results The frequency of TT,TG and GG alleles in PTO-rs17817449 were no different between MS group and non-MS group （P 〉 0.05 ）. Compared with non- MS group, the frequency of CA （ 35.6 % vs31.0% ） and AA （ 5.0 % vs 2.7% ） were significantly higher in SEC16B-rs574367 （ P 〈 0.05 ）. After adjusted gender and age in a binary logistic analysis ,the SEC16B-rs574367 was an independent risk factor for MS （OR value：1. 341,95 % CI： 1. 089-1. 651 ,P = 0. 006）. In comparison to subjects with CC allelesof SEC16B-rs574367, the 0R（95% confidence interval）was 1. 249（0. 971-1. 606）in subjects with CA alleles and was 2. 271 （1. 215-4. 247 ）in subjects with AA alleles. Conclusion SEC16B-rs574367 has a significant correlation with MS, which is a marker of genetic susceptibility for MS.
出处
《标记免疫分析与临床》
CAS
2018年第2期149-154,共6页
Labeled Immunoassays and Clinical Medicine
基金
中国航天科工集团医疗卫生科研项目(2014-LCYL-008)
