铁死亡与胶质母细胞瘤相关性探讨

Study of Correlation Between Ferroptosis and Glioblastoma

目的通过检测组织样本中谷胱甘肽过氧化物酶基因(glutathione peroxidase 4,GPX4)、溶质载体家族7成员11基因(solute carrier family 7 member 11,SLC7A11)的表达水平,探讨铁死亡与胶质母细胞瘤的相关性。方法在癌症和肿瘤基因数据库(the cancer genome atlas,TCGA)数据分析的基础上,采用实时定量PCR(Real-time PCR,RT-PCR)方法检测45例胶质母细胞瘤组织、50例低级别胶质瘤组织和20例相对正常脑组织中GPX4、SLC7A11基因的表达水平,并通过统计学方法分析不同组织间的表达差异。结果 GPX4、SLC7A11基因在胶质母细胞瘤组织中表达水平高于相对正常脑组织和低级别胶质瘤组织,经内参照归一化处理后,差异具有统计学意义(P<0.05)。结论 GPX4、SLC7A11基因作为调节铁死亡的重要基因,在胶质母细胞瘤中表达升高,铁死亡可能与胶质母细胞瘤的发生、发展相关,铁死亡相关基因或蛋白可能作为胶质母细胞瘤新的治疗靶点及治疗途径。

Objective Study the correlation between ferroptosis and glioblastoma by detecting glutathione peroxidase 4（ GPX4）and solute carrier family 7 member 11 （ SLC7A11 ）expression level in the tissues. Methods Based on the Cancer Genome Atlas（ TCGA）data analysis,expression of GPX4 and SLCTA11 gene were determined by using Real-time PCR（RT-PCR） in 45 samples of glioblastoma, 50 samples of low grade glioma and 20 samples of relative normal brain tissue. We then analyzed the different expressions of these tissues by statistic method. Results The expression levels of GPX4 and SLCTAll gene in glioblastoma tissues were considerably higher than those in relative normal cerebral tissues and low grade glioma tissues. With the normafization process, the difference is statistically significant（P 〈0.05）. Conclusion As the important gene for regulating ferroptosis, the expression of GPX4 and SLC7All gene in glioblastoma tissues were considerably high. Ferroptosis may be involved in the ttLrnorigenesis and progression of glioblastoma, so ferroptosis related genes or proteins can be used as novel glioblastoma therapeutic targets and considered as treatment pathways.