采用DVD光学头的微流细胞计的细胞分析

Cell analysis in a microfluidic cytometer applying a DVD pickup head(Translation)

一、引言流动血细胞计是当细胞通过盛满液流的设备时,单个生物细胞的物理或者生化特征被检测出来的一个系统。这些系统往往基于光学或者阻抗的测量方法,其中光学流动细胞计是最重要的商业化产品。物理参数如细胞密度或者单个细胞尺寸可以通过基于直流/低频交流阻抗测试法或者高频信号的库尔特计数器(Coulter Counter)获取。使用阻抗频谱参数仪,膜电容和细胞浆电导率等可以在细胞流经时获取。

In this paper we present an optical detection system for single biological cells that utilizes a standard DVD pickup head.In a feasibility study we have shown the detection for different particles and cells(yeast)suspended on a platinum mirror.For those experiments the integrated magnetic actuator of the pickup was used to simulate the particle movement and we found a remarkable sensitivity.Then a microfluidic flow cell with an integrated mirror was designed to be able to measure particles in a cytometric setup.The mirror at the bottom of the flow channel reflects the focused laser beam back to the integrated photodiodes.The advantage of using a DVD pickup head is not only in the low cost but also in the ability to use the integrated magnetic actuators to adjust for alignment errors between flow channel and pickup.Measurement results are given that show the high sensitivity and good repeatability for polystyrene beads and erythrocytes from cattle.In a two-parameter plot the cytometric separation of a mixture of counting beads and erythrcytes into two sub-populations is demonstrated.