氧四环素的微生物燃料电池处理及微生物群落

Oxytetracycline Wastewater Treatment in Microbial Fuel Cells and the Analysis of Microbial Communities

氧四环素(OTC)作为一种广谱性抗生素而被大量使用,其滥用不仅直接破坏生态系统,更容易引起微生物耐药性和抗性基因污染等问题.本研究利用微生物燃料电池(MFC)处理OTC,研究OTC在MFC不同运行时期的去除率变化情况,发现在运行150 d后,MFC对10 mg·L-1OTC的去除率在132 h达到99.0%.利用高通量测序技术分析并比较了原始接种猪粪与运行150 d后MFC阳极生物膜的微生物群落结构,发现厚壁菌门(Firmicutes)处于优势地位,但相比于原始接种猪粪,MFC生物膜上的变形菌门(Proteobacteria)的丰度从2.84%提高至8.92%~22.75%,此外,真细菌属(Eubacterium)的比例从几乎为0.00%显著提高至20.49%~49.00%.根据现有研究报道,Eubacterium spp.对多种氧杂环芳香族化合物具有一定的生物降解能力,本研究表明Eubacterium spp.可能是一类具有较强OTC降解能力的功能微生物.

Oxytetracycline（OTC） as an important broad spectrum antibiotic has been widely used in animal husbandry. However,the abuse of OTC not only has a direct adverse impact on ecosystems,but also exacerbates the appearance of antibiotic resistance bacteria and antibiotic resistance genes. OTC-containing wastewater was treated using microbial fuel cells（MFCs）,and the removal efficiency of OTC in MFCs in different operation periods was investigated. Result showed that the removal efficiency of 10 mg·L-1 OTC by MFCs within 132 h was up to 99. 0% after 150 d of operation. The bacterial communities in raw pig mature and anodic biofilms were studied by high-throughput sequencing. This showed that Firmicutes were both dominant on phylum a level; However,compared to the raw pig mature,the abundance of Proteobacteria greatly increased from 2. 84% to 8. 92%-22. 75% in the anodic biofilm. In addition,the abundance of Eubacterium spp. in the anodic biofilm increased obviously from nearly 0. 00% to 20. 49%-49. 00%. It has been reported that some Eubacterium spp. were able to biodegrade oxygen heterocyclic aromatic compounds contained in OTC. Therefore,Eubacterium spp. is suggested as potential functional species in the biodegradation of OTC and/or its metabolites. This work proves the feasibility and effectiveness of removing OTC-containing wastewater by using MFCs.