差示脉冲极谱法测定硝普钠溶液含量

Differential Pulse Polarographic Determination of Sodium Nitroprusside

本文建立了极谱法测定硝普钠含量的方法。采用pH7.2缓冲溶液为支持电解质溶液。以峰高为定量依据。在25～97μg·ml^(-1)浓度范围内,峰高与浓度有良好的线性关系,相关系数为:r=0.9999。方法回收率为99.39%,C.V.(%)=0.54,灵敏度达0.3μg/ml^(-1),以1M高氯酸(内含1mg·ml^(-1)K_4[Fe(CN)_6)为支持电解质,灵敏度可提高到2ng/ml。该分析方法有良好的专一性。

Sodium Nitroprusside(SNP) was determined with differential pulse polarogra-phy at a dropping mercury electrode. the pH 7.2 britton-robinson buffer was usedas supporting eletrolyte .calibration graphs were linear over the rage 25-97μg. ml^(-1)SNP the estimated detection limit was 0.3μg ml^(-1) If sample of SNP was treatedwith perchloric acid containing lmg. ml^(-1) potassium hexaeyanoferrate(II) the esti-mated detection limit was 2ng.ml^(-1) NO^+ was the determined ligand and the thera-peutically active portion of the molecule, therefore the method was selective andsenitive.