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一种快速诱变筛选雷帕霉素生产菌的方法 被引量:1

A Rapid Method of Mutation and Selection of Rapamycin-Producing Strain
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摘要 研究了一种复合诱变获得雷帕霉素生产菌——吸水链霉菌(Streptomyces hygroscopicus)高产突变株的方法。采用低剂量紫外诱变复合新型常压室温等离子体(ARTP)诱变技术构建雷帕霉素生产菌的诱变育种体系,获得高产雷帕霉素生产菌突变株,经两次复筛,效价较出发菌株提高18.4%。该方法大幅度减少了筛选量,正突变率高达70.59%,可以较短时间内提高雷帕霉素发酵单位,降低生产成本。 We studied a compound mutagenesis method to obtain a high rapamycin-producing mutant strain (Streptomyces hygroscopicus). Moreover, we constructed a mutation breeding system of rapamycin-producing strain using low dose UV mutagenesis and novel atmospheric and room temperature plasma(ARTP),and ob- tained high rapamycin-producing mutant strain,of which the titer increased by 18.4% than original strain by secondary screening. This method significantly reduces the screening quantity, the positive mutation rate can reach up to 70.59% ,improves the rapamycin fermentation unit in a short time,and reduces the production cost.
出处 《化学与生物工程》 CAS 2018年第3期28-31,共4页 Chemistry & Bioengineering
基金 河北省科技厅项目(169676404G)
关键词 雷帕霉素 吸水链霉菌 紫外诱变 常压室温等离子体诱变 正突变率 rapamycin Streptornyces hygroscopicus UV mutagenesis atmospheric and room temperature plasma mutagenesis positive mutation rate
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  • 1陈红歌,刘新育,张世敏,宋安东,贾新成.木聚糖酶高产菌株的诱变[J].微生物学通报,2004,31(6):33-36. 被引量:11
  • 2李永红,刘波,赵宗保,白凤武.圆红冬孢酵母菌发酵产油脂培养基及发酵条件的优化研究[J].生物工程学报,2006,22(4):650-656. 被引量:60
  • 3杜海英,于宏伟,韩军,李宁,贾英民.原生质体诱变选育乳糖酶高产菌株[J].微生物学通报,2006,33(6):48-51. 被引量:20
  • 4Vezina C,Kudelski A,Sehgal S N.Rapamycin (AY-22,989),a new antifungal antibiotic 1.Taxonomy of the producing streptomycete and isolation of the active principle[J].J Antibiot,1975,28(10):721.
  • 5Qi S, Xu D, Peng J,et al. Effect of tacrolimus (FK 506) and sirolimus (rapamycin) mono-and combination therapy in prolongation of renal allograft survival in the monkey[J].Transplantation,2000,69:1275-1283.
  • 6Wang J, Voutetakis A, Zheng C, Baum BJ. Rapamycin control of exocrine protein levels in saliva after adenoviral vector-mediated gene transfer[J]. Gene Ther. 2004,11(8):729-733.
  • 7Hidalgo M, Rowinsky EK. The rapamycin-sensitive signal transduction pathway as a target for cancer therapy [J]. Oncogene. 2000,19(56):6680-6686.
  • 8Stepkowski SM,Chen H,Daloze P.Rapamycin,a potent immunosuppressive drug for vascularized heart,kidney and small bowel transplantation in the rat[J].Transplantation,1991,51 (1):22-26.
  • 9Chen H,Wu J,Xu D,et al.Rapamycin reverse acute heart,kidney and pancreas allograft rejection and prevents accelerated heart allogaft rejection in the rat[J].Transplant Proc,1993,25(1):719-720.
  • 10Kahan BD,Julian BA,Pescovitz MD,et al.Sirolimus reduces the incidence of acute rejection episodes despite lower cyclosporine doses in caucasian recipients of mismatched primary renal allografts:a phase Ⅱ trial 1[J].Transplantation,1999,68 (10):1526-1532.

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