高产Monacolin K的红曲霉诱变育种

Screening of High-yield Monacolin K Strains of Monascus by Mutation Breeding

为了提高Monacolin K的产量,以紫红曲霉M2为出发菌株进行诱变育种(紫外诱变、常压室温等离子体ARTP诱变),通过构巢曲霉对峙培养、高效液相色谱HPLC等方法筛选高产Monacolin K的优秀突变菌株。结果显示:相对于原始出发菌株M2,两种方法均能够高效筛选到红曲菌株,对6株高产Monacolin K诱变菌株进行5次传代培养,发现诱变菌株产Monacolin K的能力均有下降;但紫外诱变菌株Z-4和ARTP诱变菌株M-43的Monacolin K产量分别下降2.83%和1.97%,表现为良好的遗传稳定性,说明Z-4和M-43具有潜在应用价值。

In order to improve the production of Monacolin K by Monascus,atmospheric and room temperature plasma（ ARTP） and ultraviolet（ UV） were used to induce Monascus purpureus M2. Excellent mutant strains were obtained through the screening method of plate confrontation culture of Aspergillus nidulans and high performance liquid chromatography（ HPLC）. The results showed that high-yield Monascus strains compared with its original strain M2 were obtained through mutation induced by ARTP and UV. To examine the genetic stability of six highyield mutant strains,the strains were continuously sub-cultured to the five generation. The ability to produce Monacolin K of ARTP mutant strain M-43 and UV mutant strain Z-4 fell by 1. 97% and 2. 83%,respectively. So the two mutant strains remained steady after subculture. they have potential application.