毛樱桃总黄酮对LPS诱导的RAW264.7细胞抗炎作用研究

Anti-Inflammatory Effect of Prunus tomentosa Thunb Total Flavones on LPS Induced RAW264.7 Cell Inflammation

利用LPS诱导的RAW264.7的细胞炎症模型,该研究检测了毛樱桃总黄酮对IL-6、IL-1β、PGE2生成的影响,同时,检测了毛樱桃总黄酮对COX-1和COX-2表达的影响。结果显示,当浓度为0.4、4μg/mL时,毛樱桃总黄酮对LPS诱导的RAW264.7生成IL-6、IL-1β及PGE2均有显著抑制作用(P<0.05);当浓度为40μg/mL时,毛樱桃总黄酮对LPS诱导的RAW264.7的COX-1表达无明显影响;对LPS诱导的RAW264.7的COX-2表达具有明显的抑制作用。结果表明,毛樱桃总黄酮可以显著抑制细胞炎症因子IL-6、IL-1β、PGE2的生成,并且可以明显抑制COX-2表达,而对COX-1表达影响不明显。

This work was aimed to explore the anti-inflammatory mechanism of Prunus tomentosa thunb total flavones（PTTTF） on LPS induced RAW264.7 cell inflammation. By using RAW264.7 cell inflammation model induced by LPS, the effects of PTTTF on the generation of IL-6, IL-1β, and PGE2, and the expressions of COX-1 and COX-2 were examined. The PTTTF at concentration of more than 4 μg/mL significantly inhibited the generation of IL-6, IL-1β and PGE2（P〈0.05）; whereas, when PTTTF at concentration of 40 μg/mL, it could inhibit the expression of COX-1, but not that of COX-2. PTTTF can significantly inhibit the generation of cell inflammatory factors IL-6, IL-1β and PGE2, and the expression of COX-2, but have no significant effect on the expression of COX-1.