REGγ基因下调对HaCat细胞的生物学影响

Cellular Biological Effects of REGγ Downregulation on HaCat Cells

为了研究蛋白酶体激活因子REGγ对HaCat细胞增殖、凋亡、周期的影响,该研究通过瞬时转染建立了siN、siR细胞系,并利用流式细胞术、MTT、Western blot等实验技术检测细胞的处理效果。研究结果显示,通过小干扰RNA使REGγ低表达后,HaCat细胞内抑癌基因蛋白p53、细胞周期依赖性激酶抑制因子p21表达量增加,进而抑制了细胞周期和增殖、促进了细胞凋亡。

To investigate the effects of REGγ downregulation on the cell proliferation, apoptosis and cell cycle of HaCat cells, we got the siN and siR HaCat cell lines by siRNA transfection. FACS, MTT and Western blot were used to detect the influnence of regulating REGγ expression. The results indicated that the downregulation of REGγ could elevate the expression of p53, a tumor inhibitor, and p21, a cyclin-dependent kinases inhibitors. Hence, the cell cycle and proliferation of HaCat cells were inhibited, inducing apoptosis subsequently.