精氨酸修饰的Fe_3O_4磁性纳米粒子作为基因释放载体的研究

The Fe_3O_4 nanoparticles modified by arginine as a gene release vector

基因治疗被认为是治疗目前临床实践中不能治愈的各种疾病的最有希望的策略。安全高效的基因载体是保障基因治疗成功实现的关键因素之一,本文利用磁性纳米粒子丰富的比表面积将生物安全性良好的阳离子片段与其连接,制备富有正电性表面的磁性纳米粒子作为潜在基因载体。通过将精氨酸修饰到四氧化三铁上,合成新型基因载体Arg-Fe_3O_4。重点考查Arg-Fe_3O_4的磁响应性、粒度、与DNA的相互作用、细胞毒性与基因转染效率,探讨其作为基因载体的可能性。实验结果表明,Arg-Fe_3O_4可以稳定结合并保护ct DNA,细胞毒性低,Arg-Fe_3O_4能有效将p EGFP转入Hela细胞并表达。Arg-Fe_3O_4有望发展为一种新型磁靶向性基因载体。

Gene therapy is the most promising strategy to address various diseases that in the current clinical practice are considered untreat-able. Successful gene delivery depends on the development of effective and safe delivery vectors. We modified the cationic fragments to the magnetic nanoparticles which have much more surface area. The positive nanoparticles are considered to used as potential gene vector. The Arg-Fe_3O_4 was synthesized by chemical modifying arginine( Arg) to Fe_3O_4. We had evaluated the possibility of its use as a gene carrier by studying its magnetic responsiveness,granularity,cytotoxicity and gene transfection. Results nanoparticle carrier system can protect the genes,have a high carrying efficiency and a well biocompatibility. Hence,we believe that the Arg-Fe_3O_4 nanoparticles were possible to be a potential magnetic targeting gene vector.