姜黄素对乳腺炎模型小鼠血清中抗炎因子的影响

Effect of curcumin on anti-inflammatory factors in the serum of mice mastitis model

为研究姜黄素(Curcumin)对致病性金黄色葡萄球菌(Staphylococcus aureus)的总糖浓度、总磷浓度及乳腺炎模型小鼠血清中抗炎因子的影响,采用紫外分光法和酶标比浊法测定,同时构建小鼠乳腺炎模型,用ELISA试剂盒检测各试验组中白介素6(IL-6)、白介素8(IL-8)和肿瘤坏死因子α(TNF-α)的变化情况来检测姜黄素的抗炎效果。结果表明:1)1 MIC浓度的姜黄素对菌体中总糖浓度和总磷浓度的升高趋势明显优于0 MIC浓度的姜黄素,起到显著的抑菌效果。2)姜黄素、TRSDL和环丙沙星组能显著降低乳腺炎模型小鼠血清中的IL-6、IL-8和TNF-α的浓度,而阴性对照组中这些炎性介质的浓度不断升高。本研究证实了姜黄素对Staphylococcus aureus具有明显抑制作用,且姜黄素能通过抑制乳腺炎模型小鼠血清中IL-6、IL-8和TNF-α的表达来调控小鼠内环境动态平衡。

The aim of this study was to investigate the effects of curcumin on the total sugar, total phosphorus and anti- inflammatory factors in the serum of mice with mastitis caused by Staphylococcus aureus. By using ultraviolet spectrophotometry and turbidimetry, the effect of curcumin on the determination Staphylococcus aureus total sugar and total phosphorus release were tested. At the same time, a mastitis model was built and the ELISA kit was used to detect the changes of anti-inflammatory factors including interleukin 6 （ IL-6）, interleukin 8 （IL-8） and tumor necrosis factor-α （TNF-α）. The results showed that：l）The inhibitory effect of curcumin at 1 M IC concentration on the total sugar and total phosphorus in the cell was better than that of curcumin at 0 MIC concentration displaying bacteriostatic effect. 2）The concentrations of IL-6, IL-8 and TNF-α in the serum of the mastitis model mice were significantly decreased by curcumin,TRSDL and ciprofloxacin groups, while they were increased steadily in the negative control group. The curcumin showed inhibitory effect on Staphylococcus aureus, and it regulated the homeostasis of internal environment by inhibiting the expression of IL-6, IL-8 and TNF-α in the serum of mastitis model.