2个慈竹bZIP基因的克隆、生物信息学分析及其诱导表达

Cloning and Bioinformatics Analysis of Two bZIP Genes of Bambusa emeiensis and Their Induced Expression under Abiotic Stresses

从慈竹笋转录组数据库中筛选并克隆出2个bZIP基因(BebZIP2和BebZIP6)进行生物信息学分析。分析结果表明,它们编码序列长度分别为504和720 bp,编码167个和239个氨基酸,BebZIP2和BebZIP6属于bZIP相关蛋白,与水稻OsbZIP52/RISBZ5蛋白聚在一枝。组织表达分析表明,这2个慈竹BebZIP基因在慈竹笋、茎、展开叶和未展开叶等部位均有表达,属于组成型表达,同一基因在不同组织中的表达量差异较大,表达量的大小为未展开叶>展开叶>茎>笋。对慈竹幼苗进行ABA、NaCl和PEG6000非生物胁迫处理,结果表明,BebZIP2和BebZIP6对盐、干旱和ABA胁迫均具有不同程度的响应。

According to the transeriptome data of Bambusa emeiensis shoot, two bZIP genes designated as BebZIP2 and BebZIP6 were cloned and their bioinformaties were analyzed. The bioinformaties analysis results showed that the full-length eDNA sequence of BebZIP2 and BebZIP6 were 504 and 720 bp, and encoded 167 and 239 amino acids, respectively. BebZIPs and rice OsbZIP52/RISB5 proteins were clustered into tile same branch, which were bZIP-related proteins. The tissue expression patterns of two BebZIP genes were analyzed by real-time PCR. Two BebZIP gene sequences were expressed in all shoots, stalks, unfolding leaves and rolled leaves of B. emeiensis. There were the expression differences of the same gene in the different tissues and expression quantity in the descending order was unfolding leaves, rolled leaves, stalks and shoots. The seedlings of B. emeiensis were treated with ABA, NaCI and PEG 6000 for abiotie stress analysis. The expression level of BebZIP2 and BebZ1P6 genes treated by salt, drought and ABA stresses have different degree of sensitivity.