越橘茎尖离体培养条件的研究

The in vitro Culture for Vaccinium vitis-idaea Stem Apex

为提高越橘苗木繁殖速度及苗木品质,本研究以越橘茎尖为材料,建立茎尖离体培养诱导成苗的快繁体系。结果表明:茎尖诱导最佳培养基为WPM(改良)+ZT 0.05 mg·L^(-1)+IBA 0.5 mg·L^(-1)+GA30.1 mg·L^(-1),0.1~0.3 mm长的茎尖诱导成苗率仅为28%,0.3~0.5 mm长的茎尖诱导成苗率能达到68%;茎尖长度达到0.5~0.7 mm时,茎尖诱导成苗率为79%,当茎尖长度为0.7~1.0 mm时,茎尖诱导成苗率高达94%。

In order to improve the breeding rate and qualities of Vaccinium vitis-idaea seedlings, in our study, V. vitis-idaea stem apexes were used as research materials, then rapid propagation systems of plants induced by stem apex in vitro culture was established. The results indicated that the optimal induced stem apex in vitro culture is WPM （improved） ＋ ZT 0. 05 mg·L^-1 ＋ IBA 0.5 mg·L^-1 ＋ GA30. 1 mg·L^-1. The success rate of plants induced by 0. 1 -0. 3 mm long stem apex was only 28 %, while such a rate of plants induced by 0.3 - 0. 5 mm long stem apex was up to 68% ; then stern apex reached to 0.5 -0.7 ram, the success rate was 79%, and the success rate of plants induced by 0. 7 - 1.0 mm long stem apex reached up to 94%.