百合花花青素对改善CCl_4诱导的大鼠肝纤维化作用研究

The Effect of Lilium lancifolium Anthocyanin on the Improvement of Liver Fibrosis Induced by CCl_4 in Rats

为探究百合花醇提物花青素对CCl_4所致的大鼠肝纤维化的预防、改善作用及可能机制。将32只大鼠随机分为对照组(Sham)、肝纤维化模型组(M)、百合花花青素低剂量组(LFF 0.5 g/mL)和高剂量组(LFF 1.0 g/mL)。M组、LFF组大鼠背部皮下注射50%CCl_4造模,每周2次,连续8周。LFF组大鼠给予不同剂量的百合花花青素提取物灌胃,Sham组和M组给予等体积的生理盐水,每天1次。8周后处理动物,采集血清检测ALT、AST;肝组织匀浆测定SOD、CAT、GSH-Px活力和MDA含量;Western-blot测定肝组织TGF-β1和α-SMA的表达。实验结果表明,与模型M组比较,百合花花青素能明显降低大鼠血清中ALT、AST水平;使肝组织抗氧化酶活力SOD、CAT、GSH-Px显著上升,脂质过氧化物MDA生成减少,进而诱导肝组织纤维化相关物质TGF-β1、α-SMA表达下调。百合花花青素具有良好的抗氧化活性,改善大鼠肝损伤,并逆转CCl_4诱导的肝纤维化,其机制可能与抗脂质过氧化物损伤及下调细胞外纤维组织增生有关。

In order to explore the prevention, improvement and possible mechanism of alcohol extract anthocyanin fromLilium lancifolium on the liver fibrosis induced by CCl4 in rats, thirty-two rats were randomly divided into control group（Sham）, liver fibrosis model group（M）, low dose lancifolium anthocyanin group（LFF 0.5 g/mL） and high dose lancifolium anthocyanin group（LFF 1.0 g/mL）. Rats in M group and LFF group were subcutaneously injected with 50% CCl_4 on the back, twice a week for 8 weeks. The rats in M group and LFF group were subcutaneously injected with 50% CCl_4 on the back twice a week for 8 weeks. The rats in LFF group were given different doses of alcohol extract anthocyanin fromLilium lancifolium. Sham group and M group were given the same volume of physiological saline once a day. After eight weeks, the animals were sacrificed and serum was collected for detecting alanine amiotransferase（ALT） and aspartate aminotransferase（AST）. The activities of superoxide dismutase（SOD）, catalase（CAT）, glutathione peroxidase（GSH-px） and the content of malondialdehyde（MDA） in the liver homogenate were measured. Western blot was employed to examine the expression of transforming growth factor-β 1（TGF-β1） and alpha-smooth muscle actin（α-SMA） in the liver tissue. The results showed that compared with M group, Lilium lancifolium anthocyanins could obviously reduce the ALT and AST levels in rat serum, and significantly increase the activities of SOD, CAT and GSH-Px in liver tissue, decrease the lipid peroxide MDA, and then down-regulate the expression of liver fibrosis related materials such as TGF-β1 and α-SMA. Lilium lancifolium anthocyanins had good antioxidant activity, which could significantly reverse the liver fibrosis induced by CCl_4. The mechanism might be related to resist the damage of lipid peroxide and reduce the proliferation of extracellular fibrous tissue.