人参酶解物中的皂苷成分及其对髓源抑制性细胞的作用研究

Ginsenosides of Ginseng Enzymatic Hydrolysate and Their Effects on Myeloid Derived Suppressed Cell

本文建立了同时测定人参酶解物中12种人参皂苷的高效液相色谱检测方法,探索人参提取物及酶解物主要皂苷成分差异,并以酶解前后的人参提取物作为对象,探讨其对髓源抑制性细胞(Myeloid derived suppressed cell,MDSC)的影响。人参提取物和酶解物的总皂苷含量差异不明显,而酶解物中12种人参皂苷和稀有皂苷含量(Rh1、F1、F2、Rg3、CK和Rh2)均显著高于人参提取物;稀有皂苷含量增加了4.48倍,尤其是酶解后转化生成了大量的F2和少量的F1、CK和Rh2等稀有皂苷。人参提取物及酶解物均能显著抑制MSC2细胞增殖和降低结肠癌荷瘤小鼠脾脏中的MDSC细胞比例。其中人参酶解物效果更佳,相对人参提取物,对MSC2细胞增殖抑制率提高30.00%和MDSC细胞比例降低40.50%。人参酶解物含有丰富的稀有皂苷,具有更高的生物活性,能够有效改善肿瘤微环境,从而加强了抗肿瘤能力。

A quantitative method for simultaneous determination of 12 ginsenosides in ginseng enzymatic hydrolysate was established by using high performance liquid chromatography（HPLC）. The differences of main ginsenosides between ginseng extracts and ginseng enzymatic hydrolysates were investigated., and the effects of ginseng extract on myeloid derived suppressed cell（MDSC） before and after enzymolysis was studied. The differences of total saponin content between ginseng extracts and ginseng enzymatic hydrolysates were not obvious, and the content of 12 ginsenosides and rare ginsenosides（Rh1, F1, F2, Rg3, CK and Rh2） in hydrolysates were significantly higher than those in ginseng extracts. After enzymatic hydrolysis, the content of rare ginsenosides in ginseng enzymatic hydrolysates was increased by 4.48 times, especially a large number of rare ginsenosides of F2, and a small amount of rare ginsenosides of F1, CK and Rh2 were produced. Ginseng extracts and ginseng enzymatic hydrolyzates could significantly inhibite the proliferation of MSC2 cells and reduce the proportion of MDSC cells in the spleen of colon cancer-bearing mice, and the effects of hydrolyzates were better than that of ginseng extract. Compared to ginseng extracts, the inhibition rate of ginseng enzymatic hydrolysates on MSC2 cells was increased by 30.00% and the proportion of MDSC cells was decreased by 40.50%. Consequently, the ginseng enzymatic hydrolysates were rich in rare ginsenosides and had a higher biological activity, which could effectively improve the tumor microenvironment and then enhance the anti-tumor ability.