刺五加提取物对人脐静脉内皮细胞紫外线辐射损伤的防护作用

Protective Effect of Acanthopanax Senticosus Extract on the Ultraviolet Radiation Injury of Human Umbilical Vein Endothelial Cells

研究刺五加提取物对紫外线辐射损伤人脐静脉内皮细胞(Human umbilical vein endothelial cell,HUVEC)的影响。将进入对数生长期的HUVEC细胞分为空白对照组、辐射组、给药预防组(低、中、高剂量)和给药修复组(低、中、高剂量)。各组均接受相同辐射,给药预防组提前加入不同质量浓度的刺五加提取物孵化12 h。采用MTT法检测细胞存活率,TBA法检测丙二醛(MDA)含量,WST法检测超氧化物歧化酶(SOD)水平,通过DNA-Ladder检测细胞凋亡。结果表明:经辐射后,给药预防组和给药修复组细胞存活率均有所提高,且呈质量浓度依赖性。与空白对照组相比,辐射组MDA含量升高,SOD水平下降;与辐射组比较,实验组给药后MDA含量降低,SOD水平升高。DNA-Ladder可以得到明显DNA凋亡条带。刺五加提取物能有效预防和修复辐射损伤的HUVEC细胞,且上述试验中给药预防组效果均优于给药修复组。

The effect of Acanthopanax senticosus extract on the ultraviolet radiation injury of human umbilical vein endothelial cells（HUVEC） was investigated. The HUVEC cells entering the logarithmic growth phase were divided into blank control group, radiation group, drug prevention group（low, middle and high dose） and drug repair group（low, middle and high dose）. Each group received the same radiation, and the drug prevention group was hatched for 12 h by adding different concentrations of Acanthopanax senticosus extract. The cell viability was measured by MTT assay and the content of malondialdehyde（MDA） was detected by TBA method. The level of superoxide dismutase（SOD） was detected by WST method. The apoptosis was detected by DNA-Ladder. The result showed that after radiation, the cell viability in the drug prevention group and the drug repair group were both increased in a concentration-dependent manner. Compared with the blank control group, the content of MDA in radiation group increased and the level of SOD decreased. Compared with the radiation group, the content of MDA decreased and the level of SOD increased in the experimental group. DNA-Ladder could obtain obvious DNA apoptotic bands. Acanthopanax senticosus extract could effectively prevent and repair radiation-injured HUVEC cells, and the preventive effect of the drug prevention group of the above-mentioned test was superior to the drug repair group.