基于PY-GC-MS技术对沉香挥发性成分的研究

Study on Volatile Components of Aquilaria by PY-GC-MS

本次实验着重分析沉香热解挥发组分,同时研究不同热解温度下热解挥发组分含量的差异来确定最佳热解温度。本文采用PY-GC-MS法对沉香热解成分直接进行在线分离检测,并用峰面积归一化法测定了各化学成分占总热解组分的相对含量。最终得出低温沉香热解产物较少,高温热解产物明显增多,这可能因为低温条件下沉香热解不充分挥发组分少,高温时沉香中的纤维素和木质素热解导致的。热解温度220℃时共鉴定出23种物质,其中主要组分有醛、酸和酮,除此之外基于PY-GC-MS快速检测到咖啡因、5-羟基-6,7,8-三甲基-2,3-二甲基色酮、可待因、3,5-二甲氧基-4-羟基肉桂醛、2,6-二甲氧基-4-(2-丙烯基)苯酚等物质。由此得出使用PY-GC-MS技术可在线快速检测分析沉香热解组分,并且采用不同的热解温度对其热解挥发性组分有很大的影响,在此次实验条件下沉香最佳热解温度为220℃。

This experiment was mainly focused on pyrolysis volatile components of theaquilaria, and the pyrolysis temperature was optimized using pyrolysis volatile component content as the indicator. PY-GC-MS was used to separate and detect the pyrolysis volatile components of the aquilaria online directly and the relative content of each chemical component in total pyrolysis components was determined by peak area normalization method. The results showed that there were less pyrolysis volatile components of the aquilaria at low temperature and higher pyrolysis products at high temperature, which may be due to the inadequate pyrolysis of aquilaria at low temperature and the pyrolysis of cellulose and lignin in aquilaria at high temperature. A total of 23 substances were identified（pyrolysis temperature： 220℃, of which the main components were aldehydes, acids and ketones. In addition, caffeine, 5-hydroxy-june-trimethyl-2,3-dimethyl ketone of primary colors, codeine, 3,5-dimethoxy-4-hydroxy cinnamic aldehyde and 2,6-dimethoxy-4-（2-allyl） phenol and other substances were quickly determined by PY-GC-MS. Consequently, PY-GC-MS could be used for online rapid detection and analysis of the pyrolysis volatile components in aquilaria, and pyrolysis temperature had a great influence on the components. The optimal pyrolysis temperature of aquilaria was 220℃ in.