不同锌含量Zn/PLGA/HA复合支架对人牙髓干细胞粘附能力影响的体外研究

Adhesion of Dental Pulp Stem Cells on Zn/polylactic-co-glycolic acid/hydroxylapatite Composite Scaffolds with Different Zinc Contents in Vitro

目的:制备含有不同锌含量的Zn/PLGA/HA复合支架并检测其机械性能;探讨不同锌含量的Zn/PLGA/HA复合支架在体外对人牙髓干细胞粘附能力的影响。方法:通过溶液浇铸/粒子沥滤法制备含锌质量分数0.5%、1%及2%的Zn/PLGA/HA复合支架,每组HA含量均为20%质量分数,对照组为不含锌的20%HA/PLGA支架。使用扫描电镜观察支架的表面形态,电子万能试验机检测支架的拉伸强度。将人牙髓干细胞接种在不同锌含量的Zn/PLGA/HA复合支架上培养,采用DAPI染色细胞计数法检测细胞粘附能力。结果:含锌质量分数1%和2%的Zn/PLGA/HA复合支架组拉伸强度均高于对照组(P<0.05)。扫描电镜观察到各组支架表面孔隙率均较高(87.6%),孔径158~372μm,并具有良好的孔间连通性。0.5%Zn组支架与对照组相比具有较好的促牙髓干细胞粘附能力(P<0.05)。结论:本实验中,含1%,2%Zn(wt%)的Zn/PLGA/HA支架具有较好的机械性能,含0.5%Zn(wt%)的Zn/PLGA/HA支架在体外对人牙髓干细胞粘附具有更好的促进作用,为含锌支架作为支架材料用于组织工程提供了理论基础。

Objective：To prepare Zn/polylactic-co-glycolic acid/hydroxylapatite（Zn/PLGA/HA）composite scaffolds with different zinc contents and evaluate the adhesion of dental pulp stem cells on Zn/PLGA/HA composite scaffolds.Methods：Zn/PLGA/HA composite scaffolds with different zinc contents（0.5,1 and 2 wt%）and HA（20 wt%）content were fabricated by solvent casting and particulate leaching technology.The control group was fabricated with PLGA and 20% HA without Zinc.The surface structure of the scaffolds was observed by scanning electron microscopy（SEM）.The tensile strength of the scaffolds was detected by an electro-mechanical universal testing machine.Human dental pulp stem cells were cultured with Zn/PLGA/HA composite scaffolds with different zinc contents.The cell adhesion ability was measured by DAPI staining.Results：The tensile strengths of 1%and 2%Zn groups were higher than that of control group（P0.05）.The scaffolds showed high porosity（87.6%）and the pores were highly interconnected.The pore size was 158~372μm.The cell adhesion ability in 0.5% Zn group was better than the control group（P 0.05）.Conclusion：One and two percent Zn groups show a higher tensile strength,while the adhesion ability of hDPSCs in 0.5% Zn group is better in vitro,which provides a theoretical basis for zinc-containing scaffold in tissue engineering.