摘要
目的:制备含有不同锌含量的Zn/PLGA/HA复合支架并检测其机械性能;探讨不同锌含量的Zn/PLGA/HA复合支架在体外对人牙髓干细胞粘附能力的影响。方法:通过溶液浇铸/粒子沥滤法制备含锌质量分数0.5%、1%及2%的Zn/PLGA/HA复合支架,每组HA含量均为20%质量分数,对照组为不含锌的20%HA/PLGA支架。使用扫描电镜观察支架的表面形态,电子万能试验机检测支架的拉伸强度。将人牙髓干细胞接种在不同锌含量的Zn/PLGA/HA复合支架上培养,采用DAPI染色细胞计数法检测细胞粘附能力。结果:含锌质量分数1%和2%的Zn/PLGA/HA复合支架组拉伸强度均高于对照组(P<0.05)。扫描电镜观察到各组支架表面孔隙率均较高(87.6%),孔径158~372μm,并具有良好的孔间连通性。0.5%Zn组支架与对照组相比具有较好的促牙髓干细胞粘附能力(P<0.05)。结论:本实验中,含1%,2%Zn(wt%)的Zn/PLGA/HA支架具有较好的机械性能,含0.5%Zn(wt%)的Zn/PLGA/HA支架在体外对人牙髓干细胞粘附具有更好的促进作用,为含锌支架作为支架材料用于组织工程提供了理论基础。
Objective:To prepare Zn/polylactic-co-glycolic acid/hydroxylapatite(Zn/PLGA/HA)composite scaffolds with different zinc contents and evaluate the adhesion of dental pulp stem cells on Zn/PLGA/HA composite scaffolds.Methods:Zn/PLGA/HA composite scaffolds with different zinc contents(0.5,1 and 2 wt%)and HA(20 wt%)content were fabricated by solvent casting and particulate leaching technology.The control group was fabricated with PLGA and 20% HA without Zinc.The surface structure of the scaffolds was observed by scanning electron microscopy(SEM).The tensile strength of the scaffolds was detected by an electro-mechanical universal testing machine.Human dental pulp stem cells were cultured with Zn/PLGA/HA composite scaffolds with different zinc contents.The cell adhesion ability was measured by DAPI staining.Results:The tensile strengths of 1%and 2%Zn groups were higher than that of control group(P0.05).The scaffolds showed high porosity(87.6%)and the pores were highly interconnected.The pore size was 158~372μm.The cell adhesion ability in 0.5% Zn group was better than the control group(P 0.05).Conclusion:One and two percent Zn groups show a higher tensile strength,while the adhesion ability of hDPSCs in 0.5% Zn group is better in vitro,which provides a theoretical basis for zinc-containing scaffold in tissue engineering.
出处
《口腔医学研究》
CAS
北大核心
2018年第2期169-171,共3页
Journal of Oral Science Research
基金
国家自然科学基金资助项目(编号:81570963)
关键词
锌
PLGA
HA
人牙髓干细胞
组织工程
Zinc PLGA HA Human dental pulp stem cells Tissue engineering