UPLC法测定患者美罗培南的血药浓度

Determination of Meropenem Plasma Concentration in Patients by UPLC

目的建立超高效液相色谱法测定人血浆中美罗培南的方法,并用该法对临床使用美罗培南的患者进行血药浓度测定。方法色谱柱为Zorbax Eclipse Plus C_(18)柱(2.1mm×100mm,1.8μm),流动相为乙腈-0.01mol·L^(-1)磷酸二氢钾缓冲液(6.3∶93.7,V/V),流速:0.4ml·min^(-1),柱温:30℃,检测波长:299nm,内标为甲硝唑。结果美罗培南与血浆中其它组分分离良好,线性范围为0.5~80.0μg·ml^(-1),日内和日间RSD均小于10%,美罗培南平均提取回收率为82.2%,方法平均回收率为101.9%。测定10例患者美罗培南谷浓度范围为0.73~32.30μg·ml^(-1),浓度值差异较大,其中3例患者的血药谷浓度低于4.0μg·ml^(-1)。结论该方法快速、灵敏、准确,适用于美罗培南临床血药浓度监测。临床患者美罗培南的谷浓度差异较大,测定患者美罗培南的血药浓度对促进美罗培南在临床合理应用具有重要意义。

Objective To establish a UPLC method for determining the meropenem concentration in human plasma and to apply this method to patients.Methods The samples were analyzed by an Agilent 1290 UPLC system with a Zorbax Eclipse Plus C（18） column（2.1 mm×100 mm,1.8μm）at 30℃at a flow rate of 0.4 ml·min^-1,and UV detection wavelength of 299 nm.The mobile phase was acetonitrile-0.01 mol·L^-1 KH2PO4（40∶60,V/V）.Results The calibration curve was linear within the range of 0.5-80.0μg·ml^-1 using a 0.2 ml sample volume.The intra-and inter-day precisions were both below 10%.The extraction and recovery rates were 82.2% and 101.9%,respectively.The individual variability of meropenem trough concentrations was high among the 10 patients,ranging from 0.73μg·ml^-1 to 32.30μg·ml^-1,while the concentrations in 3 patients were much lower than 4.0μg·ml^-1.Conclusion This UPLC method is sensitive,quick and accurate,which can be used for therapeutic drug monitoring（TDM）of meropenem.Determination of meropenem concentrations in patients′plasma is necessary.