复方黄栌口服液对异硫氰酸-萘酯致大鼠黄疸型肝炎的保护作用

Hepatoprotective Effect of Compound Huanglu Oral Liquid on Rat Icte rohepatitis Model by a-Naphthyl Isothiocyanate

目的观察复方黄栌口服液对异硫氰酸-萘酯致大鼠黄疸型肝炎模型的肝脏保护作用。方法将Wistar大鼠随机分为空白组,模型组,护肝宁片组,复方黄栌口服液低、中、高剂量组,连续灌胃给药10d。除空白组外,各组于实验第8天,灌胃异硫氰酸-萘酯造模,48h后处死大鼠,取少量肝组织制肝匀浆液和做病理切片,检测肝匀浆中MDA、SOD、TC、TG的活性以及肝脏病理组织学的改变为观察指标。结果与模型组相比,复方黄栌口服液低、中、高剂量均可显著降低肝脏TC、TG、MDA水平(P<0.01),可显著升高肝脏SOD水平(P<0.01,P<0.05);复方黄栌口服液低、中、高剂量的肝脏指数均显著降低(P<0.01,P<0.05);结论复方黄栌口服液对异硫氰酸-萘酯诱导的大鼠急性肝损伤具有很好的保护作用。

Objective To observe the hepatoprotective effect of compound Huangluoral liquid,which was applied to a rat model of icterohepatitis by a-naphthy1 isothiocyanate（ANIT）.Methods Wistar rats were randomly divided into blank group,model group,Huganningpian group,large,medium and small dose compound Huangluoral liquid groups.The last three groups were given by gavage compound Huangluoral liquid for 10 days.All the groups,except the blank group,were given by gavage ANIT solution to cause liver damage on the eighth day.Then after 48 h,some blood was taken from the rats and serum was separate from the blood to check the biochemical indices of activity of MDA,SOD,TC and TG in the liver homogenates and a chang of liver histopathology.Results This rat model of icterohepatitis was a success.Compared with the model group,large,medium and small doses of compound Huangluoral liquid could obviously reduce liver TC,TG and MDA（P〈0.01）.Large,medium and small doses of compound Huanglu oral liquid significantly reduced liver indexes（P〈0.01,P〈0.05）.Conclusion Compound Huangluoral liquid can obviously protect the rat liver damaged by ANIT.