摘要
目的建立同时测定天王补心片中丹参的4种主要成分(丹参酮Ⅱ_A、隐丹参酮、丹酚酸B及丹参酮Ⅰ)的UPLC-Q-TOF-MS检测方法。方法采用Waters Acquity BEH C_(18)(50mm×2.1mm,1.7μm)色谱柱,流动相为乙腈-甲酸-水体系梯度洗脱,体积流量:0.4ml·min^(-1),质谱正、负离子模式扫描,进样量:2.0μl。结果 4种成分在一定范围内均呈现良好线性关系;精密度、重复性和稳定性良好;加样回收率在99%~101%。结论所建立的方法简便、准确、快速,重复性好,可用于天王补心片中丹参酮Ⅱ_A、隐丹参酮、丹酚酸B及丹参酮Ⅰ的定量测定。
Objective To develop a UPLC-Q-TOF-MS method for the determination of four components(tanshinoneⅡA,cryptotanshinone,salvianolic acid B,and tanshinoneⅠ)in Tianwangbuxintablets.Methods The analysis was performed on a Waters Acquity BEH C18 column(50 mm×2.1 mm,1.7μm)with the mixture of acetonitrile-water-formic acid as the mobile phase,and at a flow rate of 0.4 ml·min^-1.MS scanning was performed in both a positive and negative mode.Sample volume was 2.0μl.Results The linear relationships between the concentration and peak areas of the seven compounds were all good.The average recovery(n =6)was between 99%and 101%.Conclusion This method is simple,accurate and reliable for determination of the four contents in Tianwangbuxintablets.
出处
《解放军药学学报》
CAS
CSCD
2018年第1期55-57,共3页
Pharmaceutical Journal of Chinese People's Liberation Army
基金
北京中医药大学校级课题
No.2016-JYB-JSMS-046