间隙连接蛋白37(Cx37)rs1764391基因多态性与多囊卵巢综合征不孕患者辅助生殖技术结局的关系

Correlation of Connexin 37 rs1764391 polymorphisms with assisted reproduction outcome in polycystic ovary syndrome patients

目的分析连接蛋白37(Cx37)rs1764391基因多态性在多囊卵巢综合征(PCOS)患者中对控制性超促排卵的反应性及其妊娠结局的影响。方法选择经辅助生殖技术(ART)治疗的98例PCOS不孕女性,使用Taqman技术分析rs1764391基因多态性分布,随后评估rs1764391基因型、等位基因型对超促排卵的反应性以及ART妊娠结局的相关性。结果 PCOS患者组TT基因型、等位基因T的频率(18.4%,31.6%)显著高于正常组(9.6%,P=0.03;22.3%,P=0.04);LH浓度、窦卵泡数、h CG注射日雌激素水平、获卵数在基因型为TT的PCOS患者中要显著高于其它基因型(P<0.05);此外,受精率在不同的rs1764391基因型间无统计学差异,而TT基因型患者的种植率(27.5%)与临床妊娠率(30.7%)显著低于CC、CT基因型患者(47.2%,52.3%,P=0.02;57.4%,69.6%,P=0.03);等位基因型T患者的临床妊娠率(45.1%)均显著低于C等位基因型(66.8%,P=0.04)。结论在PCOS不孕患者中Cx37 rs1764391基因多态性与ART结局密切相关。

Objective To investigate whether Connexin 37（Cx37） rs1764391 polymorphism could predict ovarian stimulation and reproductive outcomes in patients with polycystic ovary syndrome（PCOS）. Methods A total of 98 PCOS patients undergoing assisted reproductive technology（ART） were included in the present study. The genotype of rs1764391 was detected using Taqman PCR method. Then the relationship of rs1764391 polymorphism with controlled ovarian hyperstimulation, reproductive outcomes were assessed. Results The frequencies of TT genotype（18.4%） and T allele（31.6%） were significantly higher in patients with PCOS（9.6%, P=0.03; 22.3%, P=0.04）. LH concentration, estradiol level and the number of retrieved oocytes were significantly higher in PCOS patients with TT genotype when compared with other genotypes. Besides, embryo implantation rate（27.5%） and clinical pregnancy rate（30.7%） were significantly lower in PCOS patients with TT genotype than in patients with CC and CT genotypes（47.2%, 52.3%, P=0.02; 57.4%, 69.6%, P=0.03）. Similarly, the low rate of clinical pregnancy was observed in PCOS patients with T allele（45.1%） than in patients with C genotype（66.8%, P=0.04）. Conclusion In PCOS patients, Cx37 rs1764391 polymorphisms were significantly associated with reproductive outcomes.