多糖型扇贝沙司对小鼠免疫功能和抗氧化活性的影响

Effects of Scallops Polysaccharide in Scallop Sauce on the Immune Function and Antioxidant Ability in Mice

研究多糖型扇贝沙司对小鼠免疫功能和抗氧化活性的影响。以雌性昆明种小鼠为实验动物,设3个剂量组(5,10,20 mg/kg)和1个溶剂对照组(蒸馏水),连续灌胃28 d。以免疫器官指数、脾脏抗体生成细胞数、脾淋巴细胞转化试验、迟发型变态反应试验和碳粒廓清试验评价其免疫功能;以脏器指数、超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量评价其体内抗氧化活性。结果显示:多糖型扇贝沙司高剂量组可提高小鼠脾脏指数、增加脾脏抗体生成细胞数、足跖肿胀度、脾脏淋巴细胞转化功能和碳粒廓清指数;3个剂量水平均可使小鼠血清和各组织的SOD、GSH-Px活性显著增强,MDA含量降低。结论:多糖型扇贝沙司能提高小鼠的免疫功能,增强其抗氧化能力,具有一定的保健功能。

The research was aimed to study the antioxidant ability and immune function of increased scallops polysaccharide of scallop sauce. Female Kunming mice were selected as the experimental animals. The mice in three dose groups were supplied with different dosage of increased scallops polysaccharide of scallop sauce（5,10,20 mg/kg） by intragastric administration daily for 28 days. The mice in control group were given pure water. The scallops polysaccharide of scallop sauce＇s immune function was evaluated by the immune organ index, plaque forming cell（PFC） of spleen, splenic lymphocyte proliferation, delayed hypersensitivity and carbon clear test,.and the antioxidant capacity was evaluated by the index of immune organs, the activity of superoxide dismutase and glutathione peroxidase, the content of malondialdehyde.The results showed that high dose of scallops polysaccharide of scallop sauce could significantly increase the spleen index, spleen antibody forming cell number, the carbon clearance index, spleen lymphocyte transformation function and the foot swelling of mice compared with the control group. Three dose levels of scallops polysaccharide of scallop sauce could make the mice＇ the activity of SOD and GSH-Px significantly enhanced, the content of MDA decreased. The scallops polysaccharide of scallop sauce can improve the immune function of mice, enhance the antioxidation ability, and has an potential application value.