益气扶正合剂中总皂苷、总黄酮含量的测定

Content determination of total saponins and total flavonoids in Yiqi Fuzheng mixture

目的:建立益气扶正合剂(YQFZHJ)中总皂苷和总黄酮的含量测定方法。方法:采用D101型大孔树脂分离和纯化合剂中总皂苷,以黄芪甲苷为对照品,5%香草醛冰醋酸-高氯酸为显色剂,在450nm波长处测定其含量;总黄酮采用亚硝酸钠-硝酸铝-氢氧化钠法显色,以芦丁为对照品,在508nm波长处测定其含量。结果:黄芪甲苷在0.0514~0.4626mg范围内线性关系良好(r=0.9993),样品平均回收率99.72%(RSD=1.57%);芦丁在0.228~1.14mg范围内线性关系良好(r=0.9991),样品平均回收率103.36%(RSD=1.73%)。结论:该法灵敏、准确、快速,可用于益气扶正合剂的质量控制。

Objective：To investigate the method for content determination of total saponins and total flavonoids in Yiqi Fuzheng mixture. Methods ： DIO1 macroporous resin was used for the separation and purification of total saponins in the mixture, and the content of total saponins was determined at a wavelength of 450nm, with astragaloside as control and 5% vanilline - glacial acetic acid - perchloric acid as the color - developing agent. The content of totalflavonoidswas determined at a wavelength of 508nm ,with rutin as control andsodium nitrite -aluminum nitrate - sodium hydroxide asthe color - developing agent. Results ： Astragaloside showed a good linear relationship within the range of 0. 0514 ~0. 4626mg （r =0. 9993 ） ,with an average recovery rate of 99.72% （relative standard deviation RSD = 1.57% ）. Rutinshowed a good linear relationship within the range of 0. 228 -1.14mg（ r = 0. 9991 ）, with an average recovery rate of 103.36% （RSD = 1.73% ）. Conclusion：This method is sensitive, accurate,and quick and can be used for the quality control of Yiqi Fuzheng mixture.