FOXM1、hTERC与C-myc在HPV阳性宫颈癌筛查意义

The clinical significance of expression of FOXM1,hTERC or C-myc gene in cervical tissue of women with HPV-positive cervical cancer

目的:探讨FOXM1、hTERC与C-myc在HPV阳性宫颈癌筛查的临床意义。方法:选择2015年1月-2016年1月在本院妇产科接受HPV检测阳性的妇女360例,其中宫颈癌120例(宫颈癌组),CIN I-III期120例(CIN组),良性子宫病变120例(对照组)。采用免疫组织化学法,即用型免疫组化MaxVision检测FOXM1、hTERC与C-myc基因的表达。结果:3种基因的阳性表达率在正常组织、CIN组织病变及宫颈癌组织中均呈现出依次升高的特点。在宫颈癌组织中,FOXM1、hTERC与C-myc三者之间表达两两相关,而在正常宫颈组织与CIN宫颈组织中未发现相关性。FOXM1的表达与浸润深度、临床分期、分化程度及淋巴结转移有关,hTERC的表达与浸润深度、分化程度有关,C-myc的表达只与浸润深度有关(均P<0.05)。结论:FOXM1、hTERC与C-myc在宫颈癌的发生发展中可能具有重要的作用,可作为HPV阳性宫颈癌的筛查及预后评估的标志物。

Objective： To investigate the clinical significance of expression of FOXM1, hTERC or C-mye gene in screening HPV-positive cervical cancer. Methods： 360 women with positive result who tested HPV in Shiyan maternal and child health care hospital from January 2015 to January 2016 were rolled in this study. Among them, 120 women with cervical cancer were in cervical cancer group, 120 women with CIN I-III were in CIN group, and 120 women with uterine benign lesions were in control group. Immunohistoehemistry was used to detect the expression of FOXM1, hTERC or C-mye gens by MaxVision immunohistochemistry. Results： The positive rate of the three genes showed the characteristic of increasing from normal tissue to CIN tissue, and to cervical cancer. In cervical cancer, there was correlation in the expression of FOXM1, hTERC and C-myc genes, but the correlation of expression of FOXM1, hTERC and C-myc genes was not found in normal cervical tissue or cervical tissue with CIN. The expression of FOXM1 gene was related to the depth of invasion, clinical stage, differentiation degree and lymph node metastasis of cervical cancer. The expression of hTERC gene was related to the depth of invasion and differentiation of cervical cancer, and the ex pression of C-mye gene was only related to the depth of invasion of cervical cancer （P〈0.05）. Conclusion： The expression of FOXM1, hTERC and C-myc genes may play an important role in the carcinogenesis and progression of cervical cancer, which maybe develope a new idea for screening and prognosis evaluation of HPV-positive cervical cancer.