PLA@MSNs纳米粒子的制备研究

Preparation of PLA@MSNs nanoparticles

选用十六烷基三甲基溴化铵(CTAB)为模板剂,以3-氨丙基三乙氧基硅烷(APTES)为氨基化硅烷化试剂,正硅酸四乙酯(TEOS)为硅源,均三甲苯(TMB)作为扩孔剂,在80℃下合成了具有氨基修饰的二氧化硅纳米粒子。通过在酸性醇溶液中回流去除模板获得氨基化的介孔二氧化硅(MSNsNH_2)。以MSNs-NH_2为引发剂,辛酸亚锡为催化剂,在高温、氮气氛围下通过丙交酯开环聚合生成表面聚乳酸(PLA)修饰的二氧化硅纳米粒子(PLA@MSNs)。实验通过傅里叶红外光谱、激光粒度仪等验证了纳米粒子的结构、粒径分布、Zeta电位,通过噻吩蓝(MTT)法证明了其细胞毒性较低。

In this study,amino modified silica nanoparticles were synthetized at 80℃ using cetrimonium bromide（CTAB）as template,3-aminopropyltriethoxysilane（APTES）as silylating reagent,tetraethyl orthosilicate（TEOS）as silicon source,and mesitylene（TMB）as pore expanding agent.Then,amino modified mesoporous silica nanoparticles（MSNs-NH2）were obtained by refluxing in an acidic alcohol solution to remove the template.Polylactic acid modified nanoparticles（PLA@ MSNs）were prepared by ring opening polymerization of lactide under high temperature and nitrogen atmosphere using MSNs-NH2 as initiator,stannous octoate as catalyzer.The structure,particle size distribution and Zeta potential of the nanoparticles were verified by fourier transform infrared spectroscopy and laser particle size analyzer.The cytotoxicity of the nanoparticles was low evaluated by MTT method.