天麻钩藤饮加风药治疗高血压(肝阳上亢证)大鼠风药增效性研究

Research about the Synergism of Expelling Wind Drug in the Treatment of RHR(Hyperactivity of liver Yang) by Gastrodia and Uncaria Decoction add Expelling Wind Drug

目的:探讨天麻钩藤饮加风药(羌活、川芎)治疗高血压(肝阳上亢证)的增效作用及其作用机理。方法:取SD雄性大鼠130只,分为F组(假手术)、G组(正常)和造模组。造模组采用肾性高血压(RHR)大鼠模型,加灌附子汤及激惹方法,制备高血压肝阳上亢证模型,取造模成功的大鼠随机分为A组(天麻钩藤饮+风药(羌活、川芎)组)、B组(天麻钩藤饮组)、C组(卡托普利组)、D组(羌活、川芎)、E组(模型组)五组,灌胃给药4周后,检测大鼠收缩压(SBP)、血管紧张素Ⅱ(AngⅡ)、醛固酮(ALD)、C反应蛋白(CRP)等指标,评价其对RHR(肝阳上亢证)大鼠高血压的改善作用。结果:给药后,SBP:A组、B组、C组均较E组血压下降明显(P<0.05),A组比B、C组血压下降明显(P<0.05);ALD:A、B、C、D组均比E组数值低(P<0.05),A组比B、C、D组数值低(P<0.05);AngⅡ:A、B、C、D组水平较E组低,差异有统计学意义(P<0.05),D组与A组、G组差异无统计学意义(P>0.05);CRP:各组均相近,差异无统计学意义(P>0.05)。结论:天麻钩藤饮+风药(羌活、川芎)有显著的降压作用,其作用优于单用天麻钩藤饮、卡托普利,后两者降压作用相当。其降压作用可能与降低ALD、AngⅡ有关。

Objective：Study of Gastrodia and Uncaria decoction add Expelling wind drug （Notopterygium,ChuanXiong） for the treatment of hypertension （hyperactivity of liver Yang）, explore the synergism of Expelling wind drug and the mechanism. Meth- ods： 130 male SD rats were divided into F group （sham operation）,group G （normal） and the model group. The model with renal hypertension （RHR） rat model, and bowel plus irrigation Aconite Decoction method, preparation of hyperactivity of liver Yang hy- pertension model,the successful model the rats were randomly divided into five groups, namely Gastrodia and Uncaria decoction add Expelling wind drug（Notopterygium. ChuanXiong） group（group A） ,Gastrodia and Uncaria decoctiongroup（group 13）. Kato Pury group（group C） ,Expelling wind drug （Notopterygium,ChuanXiong） group（group D）, non drug group（group E）o intragastric ad- ministration after 4 weeks systolic blood pressure of rats, detection （$13P）, angiotensin II （ AngII）, aldosterone （ALD）, C reactive protein （CRP） was used to evaluate the effect of RHR on improving hypertension in rats with liver yang hyperactivity syndrome. Re- sults：After administration of SBP： A group, B group,C group compared with E group, the blood pressure decreased obviously （P〈 0.05） than the. A B C group,the blood pressure decreased obviously （P（0.05）. ALD..The level of ALD in groupA,B,C,D are all lower than group E（P〈0.05）. The level of ALD in group A is lower than group B,C,D（P〈0.05）. AngII： The level of AngII in groupA, B,C, D is lower than group E, the difference was statistically significant （P〈0.05） . D group and A group, there was no sig nificant difference in G group （ P〈 0.05 ）. CRP ： The differences among group A, B, C, D, E, F, G have no statistical significance. （ P〈 0.05）. Conclusion：Gastrodia and Uncaria decoction add Expelling wind drug （Notopterygium,ChuanXiong） can decrease the SBP of RHR（Hyperactivity of liver Yang） effectively which Gastrodia and Uncaria decoction or Kato Purygroup can not match. The lat- ter hypotensive effect is equivalent. Its antihypertensive effect may be related to the decrease of ALD,AngII.