基于细胞培养的新型微流体加热器的开发与应用

Exploitation and Application of a Novel Integrated Microfluidics Heater for Cell Cutlure

设计开发一种新型功能化的二维微流体加热器系统,用于哺乳动物细胞(Hela)的灌注培养及生长过程中的实时监控.该装置在无培养箱的条件下,可以为细胞提供生长所需的温度环境.采用聚二甲硅氧烷(PDMS)及多层控制阀门制作微流体通道,与采用镀有金和铬的玻璃片制作的加热片高度集成,构成一个加热片上具备9个加热单元的装置.通过阀门控制,装置各单元独立工作,控制各腔室细胞接种及排出.该系统可以为各腔室培养的细胞提供稳定及均匀的温度(37℃),培养结果显示所培养细胞生长状况良好,表明该装置具有较好工作性能.

A functional and robust microfluidic perfusion- based 2-D cell culture system was developed for mammalian cells （Hela） with long term monitoring, which provided a closed environment for cell growth and manipulation without the incubator. A 9-chamber poly（dimethylsiloxane） （PDMS） flow system with multi-layer quake-valve control was integrated with a microheater on an Aurum/Chromium （Au/ Cr） wafer. Based on a valve control and bypass, each chamber worked independently by feeding cells with the desired density into the chamber or chasing cells out of it. With the novel network microheater, a steady temperature of 37 ℃ was guaranteed for cells. Results show that cells grow as well as those based on the conventional tissue cultural vessels.