S1PR1/3在卵巢癌组织中的表达及其与微血管密度的关系

S1PR1/3 expression and correlation with MVD in ovarian cancer

目的:研究鞘氨醇-1-磷酸受体1/3(S1PR1/3)在卵巢癌组织中的表达,探讨S1PR1/3与卵巢癌微血管密度(MVD)的关系及临床意义。方法:选取2012~2015年于上海交通大学医学院附属仁济医院行手术治疗的患者的卵巢肿瘤石蜡标本,其中上皮性卵巢癌51例,良性卵巢肿瘤16例。免疫组化法检测卵巢肿瘤组织中S1PR1、S1PR3、CD34蛋白表达,根据CD34染色计算卵巢癌组织微血管密度(MVD)。采用Pearson相关性分析卵巢癌S1PR1/3表达与MVD的相关性。结果:卵巢癌组织中S1PR1、S1PR3表达和MVD均显著高于良性卵巢肿瘤组织,差异均有统计学意义(P=0.032,P=0.014,P=0.000)。卵巢癌组织中S1PR1表达与FIGO分期、病理级别、有无淋巴结转移及有无远处转移有关(P<0.05);S1PR3表达与FIGO分期、病理级别有关(P<0.01)。卵巢癌组织中,S1PR1和S1PR3高表达组的MVD均显著升高,差异有统计学意义(P=0.005,P=0.012)。MVD与S1PR1和S1PR3表达分别呈显著正相关(r=0.473,P=0.000;r=0.358,P=0.010)。结论:卵巢癌组织中S1PR1/3高表达,并且与肿瘤微血管形成具有重要联系,S1PR1/3有望成为判断卵巢癌预后的新指标,并为抗肿瘤治疗提供了新方向。

Objective： To investigate sphingosine-1-phosphate receptor 1/3（ S1 PR1/3）expression in ovarian cancer tissue and to explore the relationship between S1 PR1/3 and ovarian cancer angiogenesis and its clinical significance.Methods： Paraffin tissue samples were collected from surgical patients of Department of Obstetrics and Gynecology,Renji Hospital,School of Medicine,Shanghai Jiaotong University,including 16 benign ovarian tissues and 51 primary epithelial ovarian cancer（ EOC） tissues.S1 PR1,S1 PR3 and CD34 in ovarian cancer were detected by immunohistochemistry and microvascular density（ MVD） was calculated by CD34 staining.Pearson correlation analysis was used to analyze the correlation between S1 PR1/3 expression and MVD in ovarian cancer.Results： The expressions of S1 PR1、S1 PR3、MVD in ovarian cancer tissue were significantly higher than those in benign ovarian tumor（ P = 0.032,P = 0.014,P = 0.000）.S1 PR1 expression was significantly higher in patients with stage III ~ IV,highgrade group,lymph node metastasis and distant metastasis（ P0.05）.S1 PR3 expression was significantly higher in III ~ IV stage and high grade group（ P0.01）.MVD was significantly higher in S1 PR1 and S1 PR3 highly expression group（ P = 0.005,P = 0.012）.Pearson correlation analysis showed that MVD was respectively correlated with S1 PR1 and S1 PR3 expression（ r = 0.473,P = 0.000; r = 0.358,P = 0.010）. Conclusion： S1 PR1/3 is overexpressed in ovarian cancer tissues and is respectively correlated with tumor microvascular density.S1 PR1/3 expect to becomea new prognosis index of ovarian cancer and bring a new direction for anti-tumor therapy.