弓形虫棒状体蛋白及其核酸疫苗研究现状

Research status of rhoptry protein of Toxoplasma gondii and its nucleic acid vaccine

弓形虫病目前尚无理想的特效治疗药物和防治措施,研制安全便捷、保护力强的核酸疫苗是弓形虫病防治的有效策略。棒状体蛋白(Rhoptry protein,ROPs)是弓形虫在入侵宿主细胞过程中所分泌的一类蛋白,其在弓形虫入侵宿主细胞、纳虫空泡(Parsitophorous vacuole,PV)形成及胞内弓形虫的增殖调控等方面发挥着重要作用,是最具潜力的疫苗候选抗原分子之一。本文就ROPs家族重要成员特性、表达载体选择及其核酸疫苗在动物实验中的免疫原性,以及免疫保护作用等方面进行了概述。

At present, there are no ideal drugs and measures in the treatment and prevention of toxoplasmosis. The development of safe, convenient, and strong protective nucleic acid vaccine is an important strategy for prevention and control of toxoplasmosis. The rhoptry protein （ROP） is a large class of proteins secreted by Toxoplasma gondii. ROPs play an important role in the invasion of host cells, the formation of parsitophorous vacuole （PV） and the regulation of proliferation by T. gondii. Thus, ROPs become the most promising candidates of vaccine. In this paper, we summarize the important members of the ROPs, the expression vector and the immunogenicity and immunoprotection of the nucleic acid vaccine in animal experiments.