共免疫对跳蚤叮咬引起的猫过敏性皮炎保护效果评价

Evaluation of Protective Effect on Flea Allergy Dermatitis in Cats by Co-immunization of DNA and Protein

为评价共免疫r FSA1和p VAX-FSA1对于猫跳蚤过敏性皮炎(FAD)的预防免疫保护效果,用人工合成的跳蚤唾液抗原(FSA1)基因,分别构建原核表达质粒p ET28a-FSA1和真核表达质粒p VAX1-FSA1。p ET28a-FSA1转化E.coli BL21(DE3)感受态细胞,诱导表达纯化FSA1,获得r FSA1。12月龄以上猫共免疫r FSA1+p VAX-FSA1,第14天实施二免后,进行跳蚤叮咬,连续4轮,跳蚤叮咬结束后,统计皮炎评分。结果显示,第4轮攻跳蚤后共免疫r FSA1+p VAX-FSA1组的猫皮炎评分远远低于FAD对照组,说明共免疫r FSA1+p VAX-FSA1能抑制FAD症状,对跳蚤叮咬猫引起的过敏性皮炎有显著保护效果,可以作为预防猫跳蚤过敏性皮炎的良好办法。

This study evaluated the protective effect of immunoprophylaxis on flea allergy dermatitis in cats by co-immunization rFSA1 and pVAX-FSA1. The prokaryotic expression plasmids pET28a-FSA1 and eukaryotic expression plasmids pVAX1-FSA1 were constructed, respectively. PET28a-FSA1 was transformed into E. coli BL21 （DE3） competent cells to induce expression and purification of FSA1 to obtain rFSA1. More than 12 months old cats were immunized with rFSA1 ＋pVAX-FSA1, and received a second immunization after 2 weeks. After immunized, cats were challenged by fleas and sum of cat dermatitis scores were accounted for four times. The results showed that the co-immunization group dermatitis scores were very significantly lower than FAD control group at 4th flea challenge. It indicated that co-immunization of rFSAI＋pVAX-FSA1 could inhibit the symptoms of FAD, and had a significant protective effect to FAD. It could be used as a good way of anti-flea allergic diseases .