HPLC法同时快速测定人参提取物中人参皂苷的含量

Rapid Content Determination of Ginsenoside in Ginseng Extract by HPLC

目的采用HPLC法测定人参提取物中人参皂苷Rg1、Re、Rb1、Rd的含量。方法采用Agilent Poroshell120EC反相色谱柱(100mm×3.0mm,2.7μm),流动相:乙腈-水,梯度洗脱,检测波长:203nm,流速:0.75ml·min^(-1),柱温:30℃。结果人参皂苷Rg1、Re、Rb1、Rd分别在19.04~190.39μg·ml^(-1)(r=0.9999)、21.79~217.90μg·ml^(-1)(r=0.9995)、32.97~329.70μg·ml^(-1)(r=0.9998)、10.38~103.82μg·ml^(-1)(r=0.9999)范围内呈良好的线性关系。人参皂苷Rg1、Re、Rb1、Rd的平均加样回收率分别为100.8%、101.4%、99.8%、101.6%,RSD分别为1.8%、2.4%、2.1%、2.1%。结论本法简便、准确,可用于人参皂苷Rg1、Re、Rb1、Rd含量的同时测定。

Objective To establish an RP-HPLC method for the content determination of ginsenoside Rg1,Re,Rb1,Rd in ginseng extract.Methods An Agilent Poroshell 120 EC column（100 mm×3.0 mm,2.7μm）was used.A mobile phase that consisted of acetoniteile and water was programmed for gradient elution at a flow rate of 0.75 ml·min^-1.The column temperature was 30℃and the wavelength was set at 203 nm.Results The linearities of ginsenoside Rg1,Re,Rb1 and Rd were in the ranges of 19.04-190.39μg·ml^-1（r=0.9999）21.79-217.90μg·ml^-1（r =0.9995）,32.97-329.70μg·ml^-1（r =0.9998）and 10.38-103.82μg·ml^-1（r =0.9999）,respectively.The average recoveries were 100.8% ,101.4% ,99.8% and 101.6% ,respectively,with an RSD of less than 3% .Conclusion This method is simple and precise,which can be used for content determination of ginsenoside Rg1,Re,Rb1,Rd in ginseng extract.