胃肠复原膏质量标准的研究

Quality Standard for Weichang Fuyuan Ointment

目的完善胃肠复原膏的质量控制方法。方法采用TLC法鉴别方中黄芪、赤芍、蒲公英和枳壳;采用HPLC法测定处方中大黄素、大黄酚的含量。结果在TLC色谱中,可鉴别制剂中的枳壳、蒲公英、赤芍、黄芪;大黄素、大黄酚分别在1.06~53.04μg·ml^(-1)和1.72~86.08μg·ml^(-1)浓度范围内呈良好的线性关系,r值均为1.000;大黄素和大黄酚的平均加样回收率分别为101.47%、99.88%,RSD为2.00%、2.44%(n=6)。结论此方法简便、准确性好,为控制胃肠复原膏的质量提供了科学的依据。

Objective To establish a quality standard for WeichangFuyuanointment.Methods The thin layer chromatography method was used to identify astragali radix,paeoniae radix rubra,taraxaci herba and aurantii fructus.The high performance liquid chromatography method was applied to determine the contents of emodin and chrysophanol in Weichang Fuyuanointment.Results Astragali radix、paeoniae radix rubra、taraxaci herba and aurantii fructus could be indentified by TLC.Emodin and chrysophanol showed a good linear relationship in the range of 1.06-53.04μg·ml^-1 and 1.72-86.08μg·ml^-1,r =1.000 and 1.000.The average recovery of emodin and chrysophanol was 101.47% （RSD=2.00% ）and 99.88% （RSD=2.44% ）n =6,respectively.Conclusion This method is accurate and quick,which can be used for quality control of Weichang Fuyuanointment.