摘要
目的探讨不同照射方式对白细胞介素4(IL-4)诱导的RAW264.7细胞向M2型巨噬细胞极化的影响。方法以IL-4刺激RAW264.7细胞诱导其向M2型极化,在IL-4诱导细胞前、后分别进行不同剂量的照射,采用Westernblot检测不同刺激条件下RAW264.7细胞YM-1和Arg-1的表达水平,采用流式细胞术(FCM)检测不同刺激条件下RAW264.7细胞表面CD206和CD209的表达。结果不同的照射方式均上调IL-4诱导的RAW264.7细胞YM-1和Arg-1的蛋白表达水平,在IL-4诱导细胞后进行照射比在IL-4诱导细胞前进行照射显著上调细胞YM-1和Arg-1的蛋白表达;照射能抑制IL-4诱导下细胞表面CD206和CD209的表达。结论不同的照射方式促进IL-4诱导的RAW264.7细胞向M2型巨噬细胞极化,但抑制RAW264.7细胞的功能分子CD206和CD209的表达。
Objective To investigate the effect of radiotherapy on RAW264. 7 cell polarization to M2 phenotype induced by interleukin-4（ IL-4）. Methods RAW264. 7 cells were induced to polarize to M2 by IL-4. CD206 and CD209 expressions induced by different radiation condition were detected by using flow cytomtery and protein expressions of Arginase1（ Arg-1）and YM-1 of M2 phenotypes were detected by Western blot,respectively. Results Radiotherapy promoted the increase of YM-1 and Arg-1 protein expressions induced by IL-4,but downregulated CD206 and CD209 expressions. Conclusion Radiotherapy can promote the macrophage polarization to M2 induced by IL-4 but downregulated functional molecular expression.
出处
《中国辐射卫生》
2017年第6期637-641,共5页
Chinese Journal of Radiological Health
基金
山东省医学科学院医药卫生科技创新工程
山东省医药卫生科技发展项目(2015WS0199)
