脯氨酰寡肽酶对颗粒细胞孕酮合成的影响

The influence of POP on progesterone production in granulosa cells

脯氨酰寡肽酶(POP)高表达于哺乳动物卵巢,但功能不是十分清楚。本研究发现POP主要表达于小鼠卵巢颗粒细胞和膜细胞。我们培养了小鼠颗粒细胞和人来源的颗粒细胞系(KGN),利用POP特异的抑制剂KYP-2047处理颗粒细胞,发现POP对颗粒细胞的增殖没有明显影响,但KYP-2047可显著抑制了两种颗粒细胞孕酮分泌,表明POP对颗粒细胞孕酮分泌有重要调控作用。进一步研究发现POP通过调控SF1和StAR的表达参与调控颗粒细胞孕酮的合成。

POP is highly expressed in mammalian ovaries,but the function is still not clear.In this study,we found that POP was mainly expressed in granulosa cells and theca cells in murine ovaries.We cultured primary granulosa cells and human granulosa cell line（KGN）.These cells were treated with KYP-2047,one inhibitor of POP.We found KYP-2047 did not influence the cell proliferation of granulosa cells,but significantly decreased the progesterone production.Further studies showed that POP downregulated StAR expression,one key steroidogenic enzyme.It also decreased the expression of SF1,an essential regulator in the progesterone synthesis.These results suggest that POP regulates the progesterone secretion in granulosa cells by stimulating the expression of StAR and SF1.