洋甘菊多糖超声提取工艺优化及清除自由基能力研究

Optimization of ultrasonic-extraction technology and radical scavenging effect of polysaccharide from Matricaria chamomila

以水作为提取溶剂,利用洋甘菊多糖得率为指标,通过单因素试验和响应面设计试验优选超声辅助提取洋甘菊多糖最优工艺;通过测定洋甘菊多糖清除羟基自由基、DPPH的能力来评价其抗氧化活性。研究结果表明:洋甘菊多糖超声提取的最优条件为液固比为82.76 mL/g,超声时间为78.83 min,超声功率为1036.95 W,在此条件下,洋甘菊多糖得率理论值为11.20%。洋甘菊多糖对羟基、DPPH自由基均有较显著的清除作用,清除能力随着多糖质量浓度的增大而增大,表明洋甘菊多糖具有较好的抗氧化作用。

Using water as the solvent, the yield of polysaccharide as the response index, using single factor experiment, the effect of ultrasonic-assisted factors on polysaccharide from Matricaria chamomilla were designed by the response surface methodology to determined the optimum technology. The antioxidant activity of polysaccharide was determined by radical scavenging test. The experimental results showed the best extraction conditions were ： time 78.83 rain, ultrasonic extraction power 1036.95 W, the extraction solid-liquid ratio 82.76 mL/g. Under the above conditions, the yield of the crude polysaccharide was 11.20%. The polysaccharide can eliminate hydroxyl free radical and DPPH free radical, and the scavenging ability is positively related to its concentration. The conclusion is Chamomile polysaccharide has good antioxidant activity.