姜黄素对肠缺血再灌注继发性肺损伤大鼠TNF-α与IL-6表达的影响

Effects of curcumin pretreatment on TNF-α and IL-6 during lung injury induced by intestinal ischemia-reperfusion in rats

目的:观察姜黄素预处理对肠缺血再灌注继发性肺损伤大鼠TNF-α与IL-6表达的影响。方法:将3 0只清洁级大鼠随机分为3组(n=1 0):假手术组(S组),肠缺血再灌注肺损伤模型组(I/R组),姜黄素预处理组(CUR组)。CUR组大鼠于模型建立前5 d,每天胃饲姜黄素200 mg/kg,S组与I/R组胃饲等容量生理盐水。采用夹闭肠系膜上动脉75 min后恢复灌注的方法制备肠缺血再灌注继发性肺损伤模型。恢复灌注4 h后取肺组织进行肺组织损伤评分、计算肺含水率、检测TNF-α和IL-6表达水平。结果:与S组比较,I/R组和CUR组肺组织损伤评分、肺含水率、TNF-α和IL-6表达水平升高(P<0.05);与I/R组比较,CUR组肺组织损伤评分、肺含水率、TNF-α和IL-6表达水平下降(P<0.05)。结论:姜黄素预处理可减轻大鼠肠缺血再灌注继发性肺损伤,其机制可能与抑制肺组织炎症因子TNF-α和IL-6的表达相关。

Objective： To evaluate the effects of curcumin pretreatment on TNF-α and IL-6 during lung injury induced by intestinal ischemia-reperfusion in rats. Methods： Thirty female healthy SD rats were divided into three groups randomly（n=10）： sham operation group（S group）, lung injury inducing by intestinal ischemia-reperfusion group（I/R group）, curcumin pretreatment group（CUR group）. In CUR group, rats were treated intragastrically with curcumin at a dose of 200 mg/kg for 5 d before operation while rats were treated intragastrically with the same volume of NS in S group and I/R group. Lung injury induced by intestinal ischemia-reperfusion in rats was conducted by clamping the superior mesenteric artery for 75 min. The rats were sacrificed after 4-h reperfusion. The lung tissues were obtained for pathological analysis, lung water content evaluation and the determination of TNF-α and IL-6 levels. Results： The pathological scores, lung water content, and the levels of TNF-α and IL-6 were significantly increased in group I/R and group CUR as compared with group S（P〈0.05）. The pathological scores, lung water content, and the levels of TNF-α and IL-6 were significantly decreased in CUR group as compared with group I/R（P〈0.05）. Conclusion： Curcumin pretreatment attenuates the lung injury induced by intestinal ischemia-reperfusion on rats, which might be associated with inhibition of the levels of TNF-α and IL-6 in lung tissue.